LGR5启动子甲基化在胃癌中的表达

Expression of LGR5 promoter methylation in gastric cancer

目的探究富含亮氨酸的重复序列G蛋白偶联受体5(LGR5)甲基化与胃癌发生及相关临床病理参数的关系。方法利用多重PCR和Methyl Taryot技术,计算26组胃癌标本LGR5启动子甲基化水平,并与临床病理参数进行统计分析&结果LGR5甲基化水平在胃癌组织中显著降低,并且与胃癌病理分期、浸润深度、肿瘤大小、淋巴转移、1年内复发相关&结论胃癌组织中的LGR5甲基化水平有助于胃癌的诊断及对病理参数的初步判断,为预测短期复发提供参考,并可能为治疗提供新的靶点。

Objective To investigate the relationship between methylation of leucine-rich repetitive G protein-cou-pled receptor 5(LGR5)and gastric carcinogenesis and related clinicopathological parameters.Methods The methylation levels of LGR5 promoter in 26 groups of gastrie cancer specimens were caleulated by multiplex PCR and Methyl Target technique,and the clinical and pathological parameters were analyzed.Results The methylation level of LCRS was significantly decreased in gastric cancer tissues,and correlated with pathological stage,depth of invasion,tumor size,lymphatic metastasis and recurence within one year.Conclusion The methylation level of LGR5 in gastric cancer tssues is helpful for the diagnosis of gastric cancer and the preliminary judgement of patho-logical parameters.It provides a reference for redicting short-term recurrence and a new target for treatment.