摘要
基于ISSR标记技术,构建了忍冬属20个药用种质的DNA指纹图谱。PopGen32软件分析显示:20个种质的平均有效等位位点数为1.5437,Nei’s基因多样性为0.3137,Shonnon’s信息指数为0.4702,遗传一致度介于0.4565~1.0000之间;UPGMA聚类在0.735处将20个种质分成灰毡毛忍冬、忍冬和华南忍冬等3个类群。
DNA fingerprints of 20 medicinal Lonicera germplasms were constructed based on ISSR marker technology.PopGen32 software analysis showed that the average effective allele number of 20 materials was 1.5437,the genetic diversity of Nei’s was 0.3137,the Shonnon’s information index was 0.4702,and the genetic consistency was between 0.4565~1.0000.UPGMA clustering at 0.735 divided 20 materials into three groups:Lonicera japonica,Lonicera macranthoides and Lonicera confuse.
作者
曾慧杰
王晓明
蔡能
李永欣
刘思思
陈艺
王湘莹
乔中全
ZENG Huijie;WANG Xiaoming;CAI Neng;LI Yongxin;LIU Sisi;CHEN Yi;WANG Xiangying;QIAO Zhongquan(Hunan Academy of Forestry,Changsha410004,China;Hunan Key Laboratory of Tree Clones Breeding Technology,Changsha 410004,China)
出处
《湖南林业科技》
2019年第6期1-6,共6页
Hunan Forestry Science & Technology
基金
湖南省科技厅重点研发计划项目(2018NK2041)
关键词
药用忍冬
种质
分子标记
指纹图谱
medicinal Lonicera
germplasm
molecular marker
fingerprint
