左右半结肠癌与Programmed Death-1信号通路

Left and right sided colon cancer and programmed death-1 signaling pathway

目的探讨程序性杀伤因子1(PD1)信号通路对左、右半结肠癌预后的影响以及在左、右半结肠癌差异中所起到的作用。方法采用数据分析等手段分析左右半结肠癌的差异基因,分析PD1信号通路主要的PD1(PDCD1),PDL1(CD274),PDL2(PDCD1L2)在左右半结肠癌中的表达和预后差异,并采用免疫组化方法对数据分析结果进行验证。结果TCGA数据分析结果显示,左、右半结肠癌差异基因富集于免疫相关信号通路,其中包括PD1信号通路。PDCD1,CD274,PDCD1L2在右半肠癌的表达量显著高于左半肠癌。Kaplan-Meier生存分析显示,右半结肠癌总体生存率(OS)差于左半结肠癌,在PDCD1,CD274,PDCD1L2高表达组,右半结肠癌总体生存率差于左半结肠癌(P<0.05)。免疫组化结果显示PD1,PDL1在左、右半结肠癌中的蛋白表达与TCGA数据分析具有相同的结果,Kaplan-Meier生存分析结果显示PDL1的蛋白表达影响结肠癌的预后,表达量越高总体生存率越低。结论PD1/PDL1信号通路的表达差异影响左、右半结肠癌的预后。

Objective To investigate the effect of PD1/PDL1 pathway on the prognosis of left and right sided colon cancer and the role of PD1/PDL1 signaling pathway in the difference between left and right sided colon cancer.Methods The differential genes of left and right sided colon cancer were analyzed by data analysis.The expression and prognosis of PD1(PDCD1),PDL1(CD274),PDL2(PDCD1 L2)in left and right sided colon cancer were analyzed.The results of data analysis were verified by immunohistochemical method.Results TCGA data analysis showed that differentially expressed genes in left and right sided colon cancer were enriched in immune-related signaling pathways,including PD1 signaling pathway.The expression of PDCD1,CD274 and PDCD1 L2 in right sided colon cancer was significantly higher than that in left sided colon cancer.Kaplan-Meier survival analysis showed that the overall survival rate(OS)of right sided colon cancer was worse than that of left sided colon cancer.In the high expression groups of PDCD1,CD274 and PDCD1 L2,the overall survival rate of right sided colon cancer was worse than that of left sided colon cancer(P<0.05).Immunohistochemical results showed that the protein expression of PD1 and PDL1 in left and right colon cancers had the same results as TCGA data analysis.Kaplan-Meier survival analysis showed that the protein expression of PDL1 affects the prognosis of colon cancer,and the higher the overall expression The lower the survival rate.Conclusion The differential expression of PD1/PDL1 signaling pathway affects the prognosis of left and right colon cancer.