空间诱变植物乳杆菌SS18-116对高脂血症大鼠血脂的影响

Effect of Space Mutated Lactobacillus plantarum SS18-116 on Blood Lipids in Hyperlipidemia Rats

为研究空间诱变植物乳杆菌(Lactobacillus plantarum)SS18-116对高脂血症大鼠血脂的影响,将48只SPF级大鼠随机分为对照组、高脂模型组、原始菌株GS18组和SS18-116组。除对照组外,其余各组饲喂高脂饲料8 w建立高脂血症模型,期间对照组和高脂模型组灌胃生理盐水,GS18和SS18-116组按照10~9 CFU/mL以3.0 mL/kg bw连续灌胃8 w。测定大鼠血清总胆固醇(TC)、甘油三酯(TG)、低密度脂蛋白(LDL-C)、高密度脂蛋白(HDL-C)水平,并计算动脉粥样硬化指数(AI)和抗动脉粥样硬化指数(AAI)。结果表明,高脂饲养8 w后,与高脂模型组和GS18组相比,SS18-116组大鼠血清TC、TG、LDL-C均显著降低(P<0.05),AI显著降低(P<0.05),而相较于高脂模型组AAI显著(P<0.05)升高。综上所述诱变植物乳杆菌SS18-116具有显著降低高脂血症大鼠血清胆固醇和甘油三酯的功效。

To study the effects of space mutated Lactobacillus plantarum SS18-116 on blood lipid in hyperlipidemia rats,48 SPF rats were randomly divided into control group,hyperlipidemia model group,GS18-treated group and SS18-116-treated group. The SPF rats in groups except control were given high-fat diet for 8 w to establish hyperlipidemia rat model. The Wistar rats in control and hyperlipidemia model groups were administrated intragastrical with sterile normal saline,GS18 and SS18-116 groups were given continuous gavage of 8 w at 3.0 mL/kg bw at 10~9 CFU/mL. The contents of total cholesterol(TC),triglyceride(TG),low-density lipoprotein-cholesterol(LDL-C),and high-density lipoprotein-cholesterol(HDL-C)in serum of rats were determined. Atherosclerosis index(AI)and anti-atherosclerosis index(AAI)were calculated. The results showed that after 8 w of high-fat feeding,the serum TC,TG and LDL-C of the SS18-116 group were significantly reduced compared with those of the high-fat model group and the GS18 group(P<0.05). AI was significantly decreased(P<0.05),while AAI was significantly increased(P<0.05)compared with the high-fat model group. In summary,space mutated L. plantatus SS18-116 had the effect of significantly reducing serum cholesterol and triglyceride in hyperlipidemia rats.