微生物种间直接电子传递方式耦合产甲烷研究进展

Advances in co-culture stoichiometrically producing methane via direct interspecies electron transfer within microbes

厌氧产甲烷体系中细菌与产甲烷菌之间的电子传递,一直以来被认为是通过种间H2/甲酸转移来实现。然而,近年来研究发现某些电活性微生物与产甲烷菌之间存在可替代种间H2/甲酸转移以实现电子传递的种间直接电子传递(direct interspecies electron transfer,DIET)。通过DIET方式,产甲烷菌可从与其共生的微生物中直接获得的电子还原CO2产甲烷,该方式极大地提高产甲烷的效率和产甲烷的量。但是,目前对DIET方式耦合产甲烷还缺乏深入研究。文章对DIET方式耦合还原CO2产甲烷的研究现状进行了概述,重点分析了DIET方式耦合还原CO2产甲烷研究存在的问题,并讨论了其今后研究方向,以为DIET方式耦合还原CO2产甲烷研究提供参考。

Interspecies H2/formate transfer had been considered as the main pathway for electron transfer between bacteria and methanogens in anaerobic environment before. However, recent studies show that direct interspecies electron transfer(DIET) might be a potential alternative to realize electron transfer between certain electroactive microbes and methanogens. Methanogens can directly use electrons derived from its syntrophic partner to reduce carbon dioxide to methane via DIET, which greatly improves the efficiency of methane production and productivity. There is a lack of in-depth research in the study of co-culture stoichiometrically producing methane via DIET. In this review, recent progress in co-culture stoichiometrically producing methane via DIET was summarized. Some unclear key issues about co-culture stoichiometrically producing methane via DIET were discussed and several research directions for DIET were proposed with the aim of providing references for related research and development.