越橘提取物中花青素分析及其体外抗氧化活性

Analysis of Anthocyanin in Bilberry Extract and Its Antioxidant Activity in Vitro

研究越橘提取物中花青素的组成与含量及其体外抗氧化活性。实验采用高效液相色谱与质谱联用法对越橘提取物中的花青素组成进行鉴定,并以矢车菊素-3-O-葡萄糖苷为标准品测定了花青素的含量。以VC为对照,测定越橘提取物对DPPH自由基、ABTS自由基、羟基自由基的清除能力和还原力。结果表明,越橘提取物中含有14种花青素,包括飞燕草素、矢车菊素、矮牵牛素、芍药素和锦葵素类;越橘提取物中花青素的总含量为408.74μg/mg,其中,矢车菊素类花青素含量最高,为159.93μg/mg,占总含量的39.12%;体外抗氧化实验表明,越橘提取物的DPPH自由基清除能力、ABTS自由基清除能力和羟基自由基清除能力的IC50分别为26.16、13.07、1.91 mg/mL,还原力在浓度为250μg/mL时达到0.617。本研究为越橘的开发利用提供一定理论与数据基础。

The composition and content of anthocyanins in bilberry extract and its antioxidant activities in vitro were determined.The anthocyanin composition in the bilberry extract was identified by high performance liquid chromatography coupled with mass spectrometry and the content of anthocyanin was determined with cyanidin-3-O-glucoside as standard.Taking VC as control,the scavenging ability of bilberry extract to DPPH free radical,ABTS free radical and hydroxyl free radical were determined,and its reducing power and total antioxidant capacity were determined. The results showed that there were fourteen anthocyanins in bilberry extract,which contented delphinidin derivatives,cyanidin derivatives,petunidin derivatives,peonidin derivatives and malvidin derivatives. The total anthocyanins content was 408.74 μg/mg,of which the content of cyanidin was the highest,was 159.93 μg/mg,accounting for 39.12%. The antioxidant activity experiments in vitro showed that the IC50 of DPPH free radical scavenging ability,ABTS free radical scavenging ability and hydroxyl radical scavenging ability of bilberry extract were 26.16,13.07,1.91 mg/mL.The reducing power reached 0.617 when the concentration was 250 μg/mL.This study provides a certain theoretical and data basis for the development and utilization of bilberry.