磁性分子印迹纳米粒子对四环素的富集分离

Enrichment and Separation of Tetracycline by Magnetic Molecularly Imprinted Polymers Nanoparticles

以羧基化的Fe3O4纳米粒子为载体,四环素为模板分子,采用表面印迹技术制备对四环素具有特异性识别的磁性分子印迹纳米粒子。分别优化磁性分子印迹纳米粒子的制备条件和富集分离四环素的条件,为食品中四环素残留的富集分离及后续检测,提供一种简便快速的方法。结果表明,当模板分子和功能单体的摩尔比为1∶8(总体积为110 mL),羧基化Fe3O4纳米粒子的添加量为0.5 g,洗脱液甲醇-乙酸溶液的体积比为8∶2时,所制备的磁性分子印迹纳米粒子吸附性能最佳。应用最优条件制备的磁性分子印迹纳米粒子10 mg,对2 mL 0.08 mg/mL的四环素进行吸附,当反应时间为40 min时,其吸附效率可达94.10%。

The magnetic molecularly imprinted polymers nanoparticles(MMIPs NPs)were synthesized using Fe3O4 nanoparticles(Fe3O4 NPs)as carrier material,and tetracycline as template molecule based on the surface molecular imprinting technology.The synthesis conditions of MMIPs NPs were optimized,and then the application of MMIPs NPs in the enrichment and isolation of tetracycline was evaluated,which provided a simple and rapid method for enrichment,separation and subsequent detection of tetracycline.The results showed that the optimum synthesis conditions of MMIPs NPs were as follows:the mole ratio of template molecule to functional monomer was 1∶8(V:110 mL),the amount of additives of carboxyl-modified Fe3O4 NPs was 0.5 g,and the volume ratio of methanol to acetic acid solution was 8∶2.The adsorption efficiency achieved 94.10%for the tetracycline of 2 mL 0.08 mg/mL when the amount of MMIPs NPs was 10 mg,and the reaction time was 40 min.