超高压下大豆亲脂蛋白-羟丙基甲基纤维素理化性质研究

Physicochemical Properties of Soy Lipophilic Protein-Hydroxypropyl Methylcellulose Complex under Ultra-high Pressure

大豆亲脂蛋白(Lipophilic protein,LP)和羟丙基甲基纤维素(Hydroxypropyl methylcellulose,HPMC)在中性条件下(pH值7.4)复合后,可自发组成LP-HPMC复合物,但仍有部分未自组装的LP和HPMC存在于溶液中,目前仍无法实现LP-HPMC最大程度复合以及最多结合位点的目标。采用荧光光谱和红外光谱研究了不同超高压处理对LP-HPMC复合程度以及作用力的影响,采用乳化活性和乳化稳定性测定、表面疏水性分析、动态光散射粒度分析等解析了超高压对LP-HPMC结构变化与功能性质表达的构效关系。结果表明:LP-HPMC复合物通过非共价相互作用结合,蛋白二级结构发生相应改变。当压力为400-MPa时,复合物有最大的溶解度和最小的表面疏水值,分别为41.1%和57832,此时乳化活性和乳化稳定性指标也最佳。

Soy lipophilic protein(LP)and hydroxypropyl methylcellulose(HPMC)can form an LP-HPMC complex spontaneously after being combined under neutral conditions(pH value 7.4),but there are still some self-assembled LP and HPMC presented in solution.At present,the research still can not achieve the maximum compounding degree of LP-HPMC and the target of the most binding sites.Fluorescence and infrared spectroscopy were used to study the effects of different ultra-high pressure treatments on the degree of LP-HPMC compounding and the force.The effects of ultra-high pressure on structure-activity relationship of structural changes and functional properties of LP-HPMC were analyzed by emulsion activity and emulsion stability measurement,surface hydrophobicity analysis and dynamic light scattering particle size analysis.The results showed that the LP-HPMC complex was bound by non-covalent interaction and the secondary structure of the protein was changed accordingly.When the pressure was 400 MPa,the maximum solubility and minimum surface hydrophobic value of the composite were 41.1%and 57832,respectively,and the emulsification activity and emulsion stability index were also the best.