疏水性电荷诱导层析磁性微球分离鸡蛋卵清白蛋白

Separation of Ovalbumin from Egg by HCIC Magnetic Microsphere

利用疏水性电荷诱导层析磁性微球从鸡蛋中分离卵清白蛋白,优化卵清白蛋白的分离工艺.辛酸预处理pH为5.8,辛酸加入量为50μL/mL蛋清处理液,磁性微球吸附pH为5.8,洗脱pH为3.8,洗脱时加入NaCl,其浓度为1 mol/L,卵清白蛋白的回收率约为78%,表明疏水性电荷诱导层析磁性微球可以有效分离卵清白蛋白.

Ovalbumin was separated from egg through hydrophobic charge induction chromatography(HCIC)magnetic microspheres,and separation conditions were optimized.Optimal pretreatment process was pH 5.8 with caprylic acid amount of 50μL per mL egg white disposal solution.The protein was adsorbed at pH 5.8 and desorbed at pH 3.8 with addition of 1 mol/L NaCl.The recovery rate of ovalbumin was about 78%.The results suggested HCIC magnetic microspheres could efficiently separate ovalbumin from egg.