糖皮质激素受体基因(NR3C1)在肾透明细胞癌中的表达差异及意义

Expression difference and significance of glucocorticoid receptor gene(NR3C1)in renal clear cell carcinoma

目的通过数据库分析糖皮质激素受体基因(NR3C1)在肾透明细胞癌中的表达差异及意义。方法利用Oncomine数据库检索并筛选出NR3C1的信息,得到NR3C1在ccRCC与正常肾组织中的表达情况,使用GEPIA数据库分析NR3C1的表达与ccRCC患者在TCGA数据中生存率之间的关系。通过String数据库分析NR3C1基因的蛋白相互作用及上下游调控关系。结果NR3C1在ccRCC组织及正常组织中表达,差异有统计学意义(P<0.05);高表达NR3C1的患者总体生存率显著高于低表达NR3C1的患者,差异有统计学意义(P<0.05);同时,NR3C1与NCOA1、NCOA2、NOCRO1、HSP90AA1、FKBP5等蛋白存在相互作用,并且参与了炎症反应,细胞增殖和分化等生物过程。结论NR3C1在ccRCC中显著高表达,并与ccRCC的预后相关,可作为诊断和治疗的潜在靶点。

Objective To analyze the expression difference and significance of glucocorticoid receptor gene(NR3C1)in renal clear cell carcinoma.Methods The Oncomine database was used to retrieve and screen out the information of NR3C1,so as to obtain the expressions of NR3C1 in ccRCC and normal kidney tissues.The GEPIA database was used to analyze the relationship between the expression of NR3C1 and the survival rate of ccRCC patients in TCGA data.Protein interaction on one hand and upstream and downstream regulation of NR3C1 gene on the other hand were analyzed by the String database.Results The NR3C1 expression in ccRCC tissue was statistically different from that in normal renal tissue(P<0.05).The overall survival rate of the patients with a high NR3C1 expression was significantly higher than that of ones with a low NR3C1 expression(P<0.05).NR3C1 interacted with NCOA1,NCOA2,NOCRO1,HSP90AA1,FKBP5,and other proteins,and participates in inflammatory response,as well as in cell proliferation and differentiation and other biological processes.Conclusion NR3C1 is highly expressed in ccRCC and is related to the prognosis of ccRCC,so it can be used as a potential target for diagnosis and treatment.