慢性应激对大鼠海马区KYN、IDO、TRP的影响及电针的干预作用

Effect of chronic stress on KYN,IDO,and TRP in hippocampus of depression model rats and the electroacupuncture effect

目的观察慢性应激抑郁模型大鼠海马区KYN、IDO、TRP的变化以及电针的干预作用,探讨犬尿氨酸代谢途径在抑郁症的发病及治疗中的影响。方法所有大鼠在适应性饲养1周后开始实验。采用慢性应激刺激结合孤养的方式建立大抑郁模型大鼠,采用电针、氟西汀进行干预;连续21 d的慢性应激后,采用ELISA检测各组大鼠海马区TRP、IDO、KYN的变化。结果模型组大鼠敞箱实验的水平运动次数和垂直运动次数分别为(37.40±10.13)格、(4.20±2.94)次,均低于空白组(60.00±19.74)格、(16.33±2.94)次,差异均有统计学意义(均P<0.05);电针组和氟西汀组较模型组显著增加,差异均有统计学意义(均P<0.05);模型组大鼠TRP、KYN水平分别为(0.78±0.81)μg/ml、(601.23±18.13)nmol/L,均高于空白组(0.66±0.10)μg/ml、(550.18±21.30)nmol/L,IDO水平(276.33±36.05)pg/ml,低于空白组(329.89±30.11)pg/ml,差异均有统计学意义(均P<0.05);与模型组比较,电针组大鼠TRP、KYN水平降低,IDO水平升高,差异均有统计学意义(均P<0.05)。结论电针可能通过调节犬尿氨酸代谢途径的异常来达到抗抑郁作用。

Objective To observe the changes of kynurenine(KYN),Indoleamine 2,3 oxidase(IDO),and tryptophan(TRP)in chronicity stress depression model rats and the effect of electro-acupuncture(EA),and to explore the effect of kynurenine pathway in the onset and treatment of depression.Methods The study was performed after the rats were allowed to acclimate for 1 week.The chronic unpredictable mild stress and solitarily feeding method were used to make the depression models.After 21 consecutive days’chronic unpredictable mild stress,the hippocampus was collected,and the levels of TRP,IDO,KYN were detected by ELISA.Results The cross and rear scores were significantly lower in the model group than in the control group[(37.40±10.13)vs.(60.00±19.74)and(4.20±2.94)vs.(16.33±2.94);both P<0.05],and were higher in the EA group and the fluoxetine group than in the model group(all P<0.5).The levels of TRP and KYN were higherand the level of IDO was lower in the model group than in the blank group[(0.78±0.81)μg/ml vs.(0.66±0.10)μg/ml,(601.23±18.13)nmol/L vs.(550.18±21.30)nmol/L,and(276.33±36.05)pg/ml vs.(329.89±30.11)pg/ml;all P<0.05].Compared with those of the model group,the levels of TRP and KYN decreased and the level of IDO increased in the EA group(all P<0.05).Conclusion Electro-acupuncture may relieve depression by adjusting the abnormality of kynurenine pathway.