胃癌组织中lncRNA FOXF1-AS1的表达及其与临床病理特征及预后的关系

Expression of lncRNA FOXF1-AS1 in gastric cancer and its relationships with clinicopathological features and prognosis

目的:探究胃癌组织中长链非编码叉头蛋白F1-反义RNA1(lncRNA FOXF1-AS1)表达水平及其与临床病理特征及预后的关系。方法:以2014年2月至2015年7月于本院就诊胃癌患者87例为研究对象。术中取患者胃癌组织及癌旁(>5 cm)组织,荧光定量PCR检测胃癌组织及癌旁组织中lncRNA FOXF1-AS1表达水平,收集分析患者临床资料及临床病理特征,Kaplan-Meier分析胃癌患者3年生存情况,COX回归分析影响胃癌不良预后发生的危险因素。结果:胃癌组织中lncRNA FOXF1-AS1表达水平显著低于癌旁组织(P<0.05);根据lncRNA FOXF1-AS1表达平均值将患者分为高表达组和低表达组,lncRNA FOXF1-AS1表达水平与胃癌患者浸润深度、淋巴结转移、TNM分期有关(P<0.05),与患者年龄、病理类型、肿瘤直径大小无关(P>0.05);Kaplan-Meier分析结果显示lncRNA FOXF1-AS1高表达患者3年无进展生存率和总生存率显著高于lncRNA FOXF1-AS1低表达组(P<0.05);COX多因素分析结果显示肿瘤淋巴转移、lncRNA FOXF1-AS1表达水平是影响胃癌患者预后的独立危险因素(P<0.05)。结论:lncRNA FOXF1-AS1在胃癌组织中表达下调,与胃癌患者浸润深度、淋巴结转移及生存情况有关,是影响患者预后的独立危险因素,可作为患者预后判断的参考指标。

Objective:To investigate the expression of long-chain non-coding fork protein F1-antisense RNA1 in gastric cancer and its relationships with clinicopathological characteristics and prognosis.Methods:From February 2014 to July 2015,87 patients with gastric cancer in our hospital were studied.Gastric cancer tissues and adjacent(>5 cm)tissues were obtained during operation.The expression of lncRNA FOXF1-AS1 in gastric cancer tissues and adjacent tissues was detected by fluorescence quantitative PCR.The clinical data and clinicopathological features of the patients collected and analyzed.Kaplan-Meier was used to analyze the 3-year survival of patients with gastric cancer,COX regression analysis was used to analyze the risk factors of adverse prognosis of gastric cancer. Results: The expression level of lncRNA FOXF1-AS1 in gastric cancer tissues was significantly lower than that in adjacent tissues ( P <0.05).According to the average expression of lncRNA FOXF1-AS1,patients were divided into high expression group and low expression group,the expression of lncRNA FOXF1-AS1 was correlated with the depth of invasion,lymph node metastasis and TNM stage in gastric cancer patients ( P <0.05),and not with age,pathological type and tumor diameter ( P >0.05).Kaplan-Meier analysis showed that the 3-year progression-free survival rate and overall survival rate of patients with high expression of lncRNA FOXF1-AS1 were significantly higher than those with low expression of lncRNA FOXF1-AS1 ( P <0.05).COX multivariate analysis showed that lymphatic metastasis and expression of lncRNA FOXF1-AS1 were independent risk factors for prognosis of gastric cancer patients ( P <0.05). Conclusion: The expression of lncRNA FOXF1-AS1 in gastric cancer is down-regulated,which is related to the depth of invasion,lymph node metastasis and survival of patients with gastric cancer.It is an independent risk factor affecting the prognosis of patients,and can be used as a reference index for prognosis judgment of patients.