玉米生产燃料乙醇的发酵品质评价

Evaluation of Fermentation Quality of Corn for Fuel Ethanol Production

在实验室条件下模拟干法研磨工艺测定了59份玉米样品生产燃料乙醇的发酵特性探究了以淀粉含量和完全水解淀粉(CHS)含量作为评价玉米发酵品质指标的可行性采用激光扫描共聚焦显微镜(CLSM)对醪化后样品中蛋白质的微观结构进行观察研究蛋白质交联对玉米发酵转化效率的影响结果表明玉米样品的淀粉含量与乙醇产量呈极显著正相关然而淀粉含量仅能解释81%的乙醇产量差异CLSM的成像结果显示醪化后的玉米蛋白质形成致密的蜂窝状显微结构转化效率低的样品其蛋白质的蜂窝状显微结构更加致密说明蛋白质的交联程度较高CHS含量与乙醇产量之间的决定系数为0.9096用CHS含量预测乙醇产量比淀粉含量更可靠.

Fermentation properties of 59 corn samples were determined by stimulating the dry mill process in laboratory,and the feasibility of using starch content and complete hydrolyzed starch(CHS)content as indicators to evaluate fermentation quality of corn for fuel ethanol production was explored.The microstructure of protein in mashed corn samples was observed by using a confocal laser scanning microscope(CLSM)to investigate the influence of protein cross-linking on fermentation conversion efficiency.The results showed that starch content of corn samples was positively correlated to ethanol yield significantly,but it only explained 81%of the ethanol yield variation.CLSM images displayed strong web-like protein matrix in mashed corn samples,and more compact microstructures were observed in samples with lower conversion efficiencies,indicating higher degree of protein cross-linking.The coefficient of determination between CHS content and ethanol yield was 0.9096.It was more reliable for CHS content to predict ethanol yield than starch content,which was demonstrated by a validation test to a prediction model.