托盘果的功能性成分分析及其抗氧化活性研究

Study on the Functional Composition and Its Antioxidant Activity of the Fruits of Rubus crategefolius Bunge

采用水提醇沉、醇提和加热回流等方法对托盘的干燥果实进行提取,对其化学成分及抗氧化活性进行了初步研究。采用紫外可见分光光度法,测定了托盘果中总黄酮、总皂苷、总酚酸、总氨基酸、总多糖的含量,并测定了托盘果醇提取物的体外抗氧化能力。结果表明:托盘果提取物中的总黄酮、总皂苷、总酚酸、总多糖、总氨基酸含量分别为1.31%、0.96%、0.44%、0.55%、1.39%。托盘果醇提物对DPPH、ABTS、超氧阴离子和羟基自由基清除率的IC 50值分别为0.087 mg/mL、0.260 mg/mL、2.230 mg/mL、2.120 mg/mL。本试验表明托盘果提取物中含有黄酮类、皂苷类、酚酸类、氨基酸类、多糖类成分,且具有良好的抗氧化活性。

Rubus crategefolius Bunge.is used as traditional medicinal plant,and abound in natural resources.In the present study,the extract of dry fruits of R.crategefolius.Bunge was prepared by water extraction and alcohol precipitation method,alcohol extraction method,and heating reflux and other methods,and its chemical constituents and antioxidant activities were investigated.The contents of total flavonoids,total saponins,total phenolic acids,total amino acids and total polysaccharides were determined by UV spectrophotometry.The scavenging abilities of ethanol extract of R.crategefolius Bunge.fruits on DPPH free radical,ABTS free radical and superoxide anion free radical were determined.The contents of total flavonoids,total saponins,total phenolic acids,total polysaccharides and total amino acids were 1.31%,0.96%,0.44%,0.55%and 1.39%,respectively.The IC 50 values of DPPH,ABTS,superoxide anion and hydroxyl radical scavenging rate were 0.087,0.26,2.23,and 2.12 mg/mL respectively.In conclusion,fruit extract of R.crategefolius Bunge.contained flavonoids,saponins,phenolic acids,amino acids,polysaccharides,and had good antioxidant activity.