正交试验法优选菊花炭炮制工艺研究

Optimization of processing technology of charring chrysanthemum by orthogonal design

目的优选菊花炒炭的最佳工艺。方法通过L9(34)正交试验设计法制备菊花炭样品,采用高效液相色谱法测定绿原酸、木犀草苷、3,5-O-二咖啡酰基奎宁酸的含量,采用Diamonsil C18色谱柱(250 mm×4.6 mm,5μm),以乙腈为流动相A和0.1%磷酸水溶液为流动相B进行梯度洗脱;流速为1.0 mL/min,检测波长为348 nm,柱温为30℃。并进行外观性状鉴别、水分和浸出物含量测定。以外观性状、浸出物含量和绿原酸、木犀草苷、3,5-O-二咖啡酰基奎宁酸的含量综合评分,优选菊花炭的最佳炮制工艺和具体工艺参数。结果菊花的最佳炒炭工艺为:取净制菊花1000 g,置300℃热锅内,翻炒6 min。结论通过此法优选得到的菊花炒炭的工艺稳定、技术参数明确、可行性高。

Objective To optimize the processing technology of charring chrysanthemum. Methods Charred Chrysanthemum samples were prepared by means of L9(34) orthogonal test design, and the contents of chlorogenic acid, luteolin, 3,5-O-di-coffee acyl quinic acid were determined by high performance liquid chromatography(HPLC). The gradient elution was carried out with acetonitrile as mobile phase A and 0.1% aqueous phosphoric acid as mobile phase B. The flow rate was 1.0 mL/min, the detection wavelength was 348 nm, and the column temperature was 30 ℃. And the appearance character identification, the water content and the extract content determination were carried out. The optimum processing technology and the specific process parameters of charring chrysanthemum were selected based on the appearance character, the extract content and the content of the chlorogenic acid, the luteolin, the 3,5-O-di-coffee and the quinine acid. Result The optimum charring process of the chrysanthemum was as follows:1 000 g of chrysanthemum put in a hot pot with a temperature of 300 ℃, fried for 6 minutes. Conclusion The optimized technology is stable,feasible and the technical paramater is definite.