山药多糖对低强度连续微波辐射小鼠免疫系统功能的影响

Effect of yam polysaccharides on immune system function of mice exposed to continuous low-density microwave radiation

目的探究山药多糖对低强度连续微波辐射致小鼠免疫系统功能损伤的保护作用。方法将ICR雄性小鼠随机分为5组,即空白对照组、辐射损伤组及山药多糖低、中、高剂量组(200、400、800 mg/kg)。除空白对照组外,对其余各组小鼠施行10 mW/cm^2、5 min/d、连续30 d的微波辐照,建立低强度连续微波辐射损伤小鼠模型。自辐射的21 d起,各组小鼠分别给予以下处理:山药多糖低、中、高剂量组小鼠按照200、400、800 mg/kg的剂量灌胃给药,空白对照组与辐射损伤组给予等体积蒸馏水,各组均连续干预10 d。分别检测细胞免疫和体液免疫的相关指标,包括胸腺指数、脾脏指数、巨噬细胞吞噬指数、T淋巴细胞增殖率以及血清IgG、IL-4的含量。结果与空白对照组比较,辐射损伤组小鼠脾脏指数、腹腔巨噬细胞吞噬指数、T淋巴细胞增殖刺激指数及血清IgG含量下降,血清IL-4水平升高,差异有统计学意义(P<0.05,P<0.01)。与辐射损伤组比较,山药多糖不同剂量组可提高微波辐射损伤小鼠的巨噬细胞吞噬指数,T淋巴细胞增殖的刺激指数及血清IgG水平,降低血清IL-4水平(P<0.05,P<0.01)。结论山药多糖对低强度连续微波辐射引起的小鼠免疫系统功能损伤有明显改善作用。

Objective To study the protective effect of yam polysaccharides on immune system damage induced by continuous low-density microwave radiation in mice. Methods ICR male mice were randomly divided into 5 groups: blank control group, radiation injury group, and low-, medium-and high-dose yam polysaccharides groups(200, 400 and 800 mg/kg). Except for the blank control group, mice in the other groups were exposed to microwave radiation at 10 mW/cm^2, 5 min/d for 30 consecutive days to establish the mice model of continuous low-density microwave radiation. Starting from day 21 of radiation, the mice in the low-, medium-and high-dose yam polysaccharides groups were orally administered with yam polysaccharides at 200, 400 and 800 mg/kg respectively, and those in the blank control group and the radiation injury group were given distilled water of equal volume. All of the animals were treated for 10 consecutive days. Relevant indicators of cellular immunity and humoral immunity, including thymus index, spleen index, macrophage phagocytosis index, T lymphocyte proliferation rate and the serum levels of IgG and IL-4 were determined. Results Compared with the blank control group, spleen index, macrophage phagocytosis index, T lymphocyte proliferation rate and the serum level of IgG decreased and the serum level of IL-4 increased in the radiation injury group, with significant differences(P<0.05, P<0.01). Compared with the radiation injury group, macrophage phagocytosis index, T lymphocyte proliferation rate and the serum level of IgG increased and the serum level of IL-4 decreased in the yam polysaccharides groups(P<0.05, P<0.01). Conclusion Yam polysaccharides significantly improve the impaired immune system function induced by continuous low-density microwave radiation in mice.