外周血T淋巴细胞亚群在不同致敏原因哮喘儿童中的表达研究

Expression of peripheral blood T lymphocyte subsets in children with different sensitization causes

目的 探究外周血T淋巴细胞亚群在不同致敏原因哮喘儿童中的表达,旨在为临床的诊断和治疗提供科学依据。方法 选取2016年4月至2018年4月于我院接受治疗的84例哮喘儿童为研究对象,根据致敏原因将所有患者分为两组,其中观察组为多重致敏,共43例患者,对照组为单一致敏,共41例,比较两组患者的CD4^+细胞和CD8^+细胞及两者的比值、Th1细胞和Th2细胞及两者的比值、Tregs细胞和Th17细胞及两者的比值。结果 观察组患者的CD4^+细胞高于对照组患者,CD8^+T细胞低于对照组患者,且两者比值更大,Th1细胞和Th2细胞及两者比值差别不大,Tregs细胞数量差别不大,但Th17细胞高于对照组,比值低于对照组。观察组患者CD4^+细胞为35.12±10.22%,CD8^+T细胞为22.79±8.74%,CD4^+/CD8^+T为1.43±0.24,Th1细胞为9.03±4.95%,Th2细胞为8.69±5.03%,Th1/Th2为1.02±0.08,Tregs细胞为2.11±0.43%,Th17细胞为1.31±0.72%,Tregs/Th17为1.58±0.29;对照组患者CD4^+细胞为32.03±10.12%,CD8^+T细胞为25.83±9.17%,CD4^+/CD8^+T为1.15±0.31,Th1细胞为9.11±4.88%,Th2细胞为8.71±4.95%,Th1/Th2为1.01±0.07,Tregs细胞为2.08±0.39%,Th17细胞为0.92±0.45%,Tregs/Th17为2.03±0.55。结论 外周血T淋巴细胞亚群在不同致敏原因哮喘儿童中的表达略有不同,其中CD4^+与CD8^+T比值更高,Th1细胞与Th2细胞比值差别不大,Tregs与Th17比值更低。

Objective To explore the expression of peripheral blood T lymphocyte subsets in children with different sensitization causes,aim to provide a scientific basis for clinical diagnosis and treatment.Methods Eighty-four children with asthma who were treated in our hospital from April 2016 to April 2018 were enrolled.All patients were divided into two groups according to the cause of sensitization.Among them,the observation group was multiple sensitization,a total of 43 patients,the control group was single consistent,a total of 41 cases.The ratio of CD4^+ T cells and CD8^+ T cells and the ratio of the two groups,the ratio of Th1 cells and Th2 cells and the ratio of the two groups,the ratio of Tregs and Th17 cells,and the ratio between the two were compared.Results The CD4^+ T cells of the observation group were higher than those of the control group,and the CD8^+ T cells were lower than the control group,and the ratio of the two was larger.There was no significant difference in the ratio of Th1 cells and Th2 cells,and the number of Tregs cells was not much different,but Th17 cells were higher than the control group,and the ratio was lower than that of the control group.The CD4^+ T cells in the observation group were 35.12±10.22%,the CD8^+ T cells were 25.83±9.17%,and the CD4^+ /CD8^+ was 1.43±0.24.Th1 cells were 9.03±4.95%,Th2 cells were 8.69±5.03%,and Th1/Th2 was 1.02±0.08.Tregs cells were 2.11±0.43%,Th17 cells were 1.31±0.72%,and Tregs/Th17 were 1.58±0.29.In the control group,CD4^+ T cells were 32.03±10.12%,CD8^+ T cells were 22.79±8.74%,and CD4^+ /CD8^+ was 1.15±0.31.Th1 cells were 9.11±4.88%,Th2 cells were 8.71±4.95%,and Th1/Th2 was 1.01±0.07.Tregs cells were 2.08±0.39%,Th17 cells were 0.92±0.45%,and Tregs/Th17 were 2.03±0.55.Conclusion The expression of T lymphocyte subsets in peripheral blood was slightly different in children with different sensitization causes.The ratio of CD4^+ to CD8^+ was higher,the ratio of Th1 cells to Th2 cells was not much different,and the ratio of Tregs to Th17 was lower.