HPLC-DAD法同时测定三黄洗剂中5种成分的含量

Simultaneous determination of the content of five ingredients in Sanhuang Lotion by HPLC-DAD

目的:建立高效液相色谱法(HPLC)同时测定三黄洗剂中5种成分(盐酸小檗碱、黄芩苷、汉黄芩苷、芦荟大黄素和大黄酸)的含量。方法:采用ZORBAX Eclipse XDB-C18色谱柱(4.6 mm×250 mm,5μm);甲醇-0.1%磷酸溶液为流动相梯度洗脱,流速1.0 mL/min;检测波长265 nm;柱温30℃;进样量10μL。结果:盐酸小檗碱、黄芩苷、汉黄芩苷、芦荟大黄素和大黄酸分别在28.56~171.36μg/mL,27.26~163.56μg/mL,8.84~53.04μg/mL,1.508~9.048μg/mL,0.642~3.852μg/mL(r均≥0.999 8)范围内呈良好的线性关系,平均加样回收率(n=6)及相应的RSD分别为98.70%(1.60%),98.61%(1.72%),99.09%(1.97%),98.16%(4.23%),99.71%(2.84%)。结论:该实验建立的高效液相色谱法简便快速,结果准确,稳定性良好,为科学评价和有效控制三黄洗剂的质量提供可靠依据。

Objective:To develope a(HPLC)method for simultaneous determination of the content of five ingredients in Sanhuang Lotion,including berberine hydrochloride,baicalin,wogonoside,aloe emodin,rhein.Methods:The analysis was performed on an Agilent Eclipse XDB-C18(4.6 mm× 250 mm,5 μm)chromatographic column.The mobile phase was gradient elution of methanol and 0.1% formic acid aqueous solution at a flow rate of 1.0 mL/min.The detection wavelength was 265 nm and column temperature was 30 ℃,and the injection volume is 10 μL.Results:Berberine hydrochloride,baicalin,wogonoside,aloe emodin,rhein showed good linear relationships within the range of 28.56~171.36 μg/mL,27.26~163.56 μg/mL,8.84~53.04 μg/mL,1.508~9.048 μg/mL,0.642~3.852 μg/mL(all r≥0.999 8),the average recoveries were 98.16%~99.71% with the RSD of 1.60%~4.23%.Conclusion:The HPLC method established in this experiment was simple,quick,accurate and stable.The method can provide a reliable basis for scientific evaluation and effective control of the quality of Sanhuang Lotion.