“全红”和“粉玉”瓯江彩鲤体表组织中差异色素的鉴定

Identification of differential pigments in Whole Red and Whole White coloration of Oujiang color common carp(Cyprinus carpio var. color)

为了解“全红”和“粉玉”瓯江彩鲤(Cyprinus carpio var.color)体表组织中的差异色素,分别采用分光光度法和高效液相色谱方法对两种体色瓯江彩鲤的皮肤、鳍条和眼睛中的差异色素进行鉴定。分光光度法测定结果表明:“全红”皮肤中类胡萝卜素含量显著高于“粉玉”皮肤(P<0.05),而蝶啶色素不存在显著性差异(P>0.05)。随后通过高效液相色谱法对这两种体色的皮肤、眼睛和鳍条中类胡萝卜素的种类和含量进行鉴定,发现“全红”的皮肤、眼睛和鳍条中含有大量的以酯化形式存在的虾青素和黄体素,以及部分未知的类胡萝卜素,而“粉玉”的这些组织中检测不到虾青素和黄体素等类胡萝卜素。“全红”鳍条中虾青素含量最高为(28.00±3.61)mg/kg,而皮肤中的黄体素含量最高为(62.34±4.93)mg/kg。研究表明:虾青素和黄体素等类胡萝卜素是瓯江彩鲤红色体色的关键色素,蝶啶色素对红色没有作用,瓯江彩鲤白色体色存在类胡萝卜素的缺失现象。

The spectrophotometry and high performance liquid chromatography(HPLC)methods were used to identify the different pigments between whole red(WR)and whole white(WW)coloration of Oujiang color common carp(Cyprinus carpio var.color).The results of spectrophotometry methods showed the concentration of carotenoids in WR skins were significiantly higher than that in WW skins(P<0.05),bur no difference of pteridine was found between WR and WW skins(P>0.05).Then,the HPLC results revealed that a large mounts of astaxanthin,lutein,and several unknown carotenoids were detected in the eyes,skins and fins in WR,but not detected in WW tissues.The highest concentration of astaxanthin was found in WR fins[(28.00±3.61)mg/kg],and the highest concentration oflutein was found in WR skins[(62.34±4.93)mg/kg].These results indicated that the carotenoids play a key role in the red coloration formation in Oujiang color common carp,the white color is caused by the deficiency of carotenoids,and the pteridines may have no effects on the red coloration formation in Oujiang color common carp.This study provides insight into white and red coloration formaiton in Oujiang color common carp.