水稻‘一目惚’抵御盐碱胁迫部分相关基因表达

Expression of Some Genes Related to Resistance to Salt-alkali Stress in’Hitomebore’

在本研究中,采用三种不同浓度(0.075 mol/L,0.05 mol/L,0.0375 mol/L)盐碱溶液胁迫水稻优良品种‘一目惚’,并通过表型观测及实时荧光定量PCR方法对植株进行验证分析。表型观测结果显示盐碱胁迫对于‘一目惚’的正常生长都有损伤,主要表现为叶片发黄及植株矮小,且相对来说碱胁迫损伤程度更大。但0.0375 mol/L浓度盐胁迫的植株在恢复生长7 d后生长态势与正常生长植株差异较小,推测该浓度处于‘一目惚’可应答胁迫范围内。在碱胁迫组中,植株的根部生长较为旺盛,且根长与胁迫浓度呈正相关。基因表达检测结果显示,基因OsAMT1;1和同家族基因OsAMT3;2在受胁迫植株中表达量增加,OSAMT基因家族与铵离子转运相关,表达上调表明氮代谢过程受到促进,植株以此抵御盐碱逆境;基因OsCBL4在受胁迫植株中表达下调,其作为Ca2+相关因子,在逆境来临时,形成盐碱耐受因子,以此减少盐碱逆境对植物代谢的伤害,具体分子机制还需要进一步研究。本研究创新性地采用优良耐盐碱品种‘一目惚’,进行验证性分析该品种的耐性研究,旨在提供一定的耐盐碱数据支持,为深入研究植物耐盐碱机制提供一定的实验帮助。

In this study,three saline-alkali solutions(0.075 mol/L,0.05 mol/L and 0.0375 mol/L)were used to stress the superior variety’Hitomebore’,and the plants were tested and analyzed by phenotypic observation and real-time fluorescence quantitative PCR.Phenotypic observations showed that saline-alkali stress damaged the normal growth of’Hitomebore’,mainly for yellowing leaves and dwarfing plants,and relatively more damage was caused by alkali stress.However,after 7 days of the resumption of growth,the growth situation of 0.0375 mol/L salt-stressed plants was slightly different from that of plants that grew normally.It is presumed that the concentration is within the range of’Hitomebore’to respond to the stress.In the alkali-stress group,the growth of the plants’roots was vigorous,and the length of roots was positively correlated with the stress concentrations.The test results of gene expression showed that OsAMT1;1 gene and OsAMT3;2 gene increased gene expression in stressed plants,and OSAMT gene family were related to ammonium ion transport.Up-regulation gene expression of OSAMT gene family indicated that nitrogen metabolism was promoted to resist saline-alkali stress.OsCBL4 gene was down-regulated in stressed plants.As a Ca2+related factor which formed saline-alkali tolerance factor when stress came,OsCBL4 gene could reduce sainet-alkali stress.The specific molecular mechanism of environmental damage to plant metabolism need further study,but the specific molecular mechanism needs further study.In this study,we innovatively used the excellent saline-alkali tolerant variety’Hitomebore’,to verify and analyze the tolerance of this variety,in order to provide certain data support for saline-alkali tolerance,and to provide some experimental help for in-depth study of plant saline-alkali tolerance mechanism.