木薯MeCPN20基因的克隆及其干旱胁迫下的表达分析

Gene Cloning of MeCPN20 in Cassava and Their Expression Analysis under Drought Stress

CPN20不仅是CPN60的共伴侣蛋白,可以辅助CPN60帮蛋白质进行正确折叠,还具有多种独立于分子伴侣之外的功能。为了研究木薯中CPN20基因的分子功能,利用同源克隆的方法从木薯品种‘新选048’中克隆到CPN20基因,命名为MeCPN20,该基因的cDNA全长为1101 bp,其中开放阅读框(open reading frame,ORF)为771 bp,编码256个氨基酸。生物信息学分析表明,MeCPN20蛋白含有2个串联的cpn10结构域,与同属大戟科的橡胶树、蓖麻和麻风树的CPN20蛋白亲缘关系较近,同源性分别为92%、89%和89%。荧光定量PCR分析表明,随着干旱程度的增加,MeCPN20基因的表达量呈逐渐上升的趋势。本研究结果为后期深入分析MeCPN20基因的功能提供理论基础。

CPN20 has some other fu nctions independent of its co-chaperonin role,which helps CPN60 to fold proteins correctly.In order to study the molecular function of CPN20 gene in cassava,a CPN20 gene was cloned from’Xinxuan 048’,named MeCPN20,and the total length of cDNA was 1101 bp in which the open reading frame(ORF)was 771 bp and 256 amino acids were encoded.Bioinformatics analysis showed that there were two homologous cpn10 domains in MeCPN20 protein.MeCPN20 protein was closely related to the CPN20 protein of the Hevea brasiliensis,Jatropha curcas and Ricinus communis,and the homology was 92%,89%and 89%,respectively.The q RT-PCR results indicated that the expression of MeCPN20 gene increased gradually with the increase of drought intensity.The results of this study will provide a theoretical basis for in-depth analysis of the function of MeCPN20 gene in later stage.