高粱MADS-box家族基因的鉴定与分析

Identification and Analysis of MADS-box Gene Family of Sorghum Bicolor

MADS-box家族是一类具有多种生物功能的转录因子,它参与了植物生长发育的各个过程。本研究利用生物信息学方法,从高粱全基因组数据库中筛选并鉴定出98个高粱MADS-box家族成员,并对该家族成员的蛋白理化性质、系统进化树、染色体定位、基因结构、基因表达模式等特征进行了全面分析。结果表明98个高粱MADS-box基因包含了42个M-type-MADS基因和56个MIKC-MADS基因。高粱MADS-box家族基因的内含子数目为0~10个不等,不同基因的内含子数目相差很大。这些基因位于1~10号染色体,且分布较为均匀。高粱花发育过程中有28个M-type-MADS基因和55个MIKC-MADS基因转录表达。果实发育过程中有32个M-type-MADS基因和54个MIKC-MADS基因转录表达。

The MADS-box genes family is a class of transcriptional factor with multi-functional, widely involved inmany processes during plant growth and development. In this study, a total of 98 MADS-box genes were identifiedin Sorghum bicolor with bioinformatics methods. And then physico-chemical properties of proteins, phylogenetic re-lationship, chromosome location, gene structure and gene expression pattern of the MADS-box TFs family in Sor-ghum bicolor were analyzed. The results showed that the 98 MADS-box genes contained 42 M-type-MADS genesand 56 MIKC-MADS genes. The number of introns in MADS-box family genes varied from 0 to 10. And there weregreat differences in number of introns in different genes. These genes were evenly distributed on chromosomes 1 to10. During flower development, 28 M-type-MADS genes and 55 MIKC-MADS genes were expressed. 32 M-type-MADS genes and 54 MIKC-MADS genes were expressed during fruit development.