miR-199a-3p对Th17细胞分化的调节及对重症支原体肺炎的影响

Regulation of miR-199a-3p on Th17 cells differentiation and the effect on severe mycoplasmal pneumonia

目的探讨miR-199a-3p在儿童重症支原体肺炎(MP)中的作用,并研究其对Th17细胞的影响。方法选取60例重症期MP患儿(重症组),50例恢复期MP患儿(恢复组)和40名健康儿童(对照组)为研究对象。采集所有研究对象的静脉血样本,使用逆转录聚合酶链式反应(RT-PCR)检测外周血白核细胞中miR-199a-3p的表达。采用酶联免疫吸附测定(ELISA)法检测血清中白细胞介素(IL)-17a、IL-13、粒细胞-巨噬细胞集落刺激因子(GM-CSF)和IL-6的含量。通过体外定向诱导分化Th17细胞并转染miR-199a-3p模拟物或抑制物,研究miR-199a-3p对Th17细胞分化的影响。结果与对照组相比,重症MP患儿外周血白细胞中miR-199a-3p的表达量明显降低(P<0.05)。血清中IL-17a、IL-13、GM-CSF和IL-6的含量明显高于对照组(均P<0.05)。miR-199a-3p的高表达抑制了Th17细胞的体外分化。结论miR-199a-3p的表达量在MP发病过程中降低;Th17细胞作为miR-199a-3p的靶细胞,其分化受到miR-199a-3p的调控;miR-199a-3p的高表达明显抑制了Th17细胞的分化。

Objective To investigate the role of miR-199a-3p in children with severe mycoplasmal pneumonia(MP)and to study its effect on Th17 cells.Methods Sixty children with severe MP(severe group),50 children with recovery MP(recovery group)and 40 healthy children(control group)were enrolled.Venous blood samples were collected from all subjects,and the expression of miR-199a-3p in the peripheral blood leukocytes was detected by RT-PCR.The levels of IL-17a,IL-13,GM-CSF and IL-6 in serum were determined by enzyme-linked immunosorbent assay(ELISA).The effect of miR-199a-3p on the differentiation of Th17 cells was investigated by inducing differentiation of Th17 cells and transfecting miR-199a-3p mimics or inhibitors in vitro.Results Compared with the control group,the expression of miR-199a-3p in peripheral blood leukocytes of children with severe MP was significantly decreased(P<0.05).The levels of IL-17a,IL-13,GM-CSF and IL-6 in serum were significantly higher than those in the control group(all P<0.05).High expression of miR-199a-3p inhibited the differentiation of Th17 cells in vitro.Conclusions The expression of miR-199a-3p is decreased in the pathogenesis of MP.Th17 cells are the target cells of miR-199a-3p,and their differentiation is regulated by miR-199a-3p.The high expression of miR-199a-3p significantly inhibites the differentiation of Th17 cells.