基于数据库分析HK2基因在肾透明细胞癌中的表达及临床意义

Discovery of the expression of HK2 gene in clear cell carcinoma of the kidney and its clinical significance based on database analysis

目的利用数据库和生物信息学的方法分析己糖激酶2(HK2)基因在肾透明细胞癌患者中病理特征和预后相关性。方法采用Oncomine数据库分析肾透明细胞癌和非肾组织差异最显著的基因,使用GPEIA、The Human Protein Atlas数据库检验mRNA和蛋白质的表达水平,使用LinkedOmics、KM-plotter数据库分析其表达水平与肾透明细胞癌临床病理特征和预后的相关性,使用STRING数据库预测其潜在作用的蛋白质,推测其作用机制。结果HK2为差异最显著的基因,其在肾透明细胞癌组织中高表达,并且与肾细胞癌的病理分期、T分期、N分期均呈正相关性(均P<0.05),高表达HK2的肾透明细胞癌患者总生存率显著低于低表达者(P<0.05)。结论HK2基因高表达可能与肾透明细胞癌病理分期、高T分期、高N分期和不良预后相关。

Objective To analyze the association between hexokinase 2(HK2)gene expression and clinical pathological characteristics in renal clear cell carcinoma using database and bioinformatics methods.Methods Oncomine database was used to analyze the most significant differentially expressed genes between renal clear cell carcinoma and non-kidney tissue.The expression levels of mRNA and protein were detected by GPEIA and The Human Protein Atlas database.Correlation of HK2 expression level between clinicopathological features and prognosis of renal clear cell carcinoma was analyzed using LinkedOmics and KM-plotter databases,respectively.The STRING database was used to predict the potential protein interaction mechanism.Results The most significant difference protein in renal clear cell carcinoma,i.e.HK2,was found,which was highly expressed in renal clear cell carcinoma tissues,and positively correlated with pathological stage,T stage and N stage of renal cell carcinoma(all P<0.05).The overall survival rate of the renal clear cell carcinoma patients with high expression of HK2 was significantly lower than that of the patients with low expression(P<0.05).Conclusions High expression of HK2 gene may be associated with pathological staging,high T stage,high N stage,and poor prognosis of renal clear cell carcinoma.