大蒜醇提液对小鼠离体小肠平滑肌运动的影响

Effect of Alcohol Extract from Garlic on the Motility of Murine Small Intestinal Smooth Muscle in Vitro

【目的】研究大蒜醇提液对胃肠运动的影响,了解其可能的作用机制。【方法】用生物信号处理系统监测大蒜醇提液对小鼠离体小肠平滑肌运动的影响。【结果】2倍浓度递增(0.025%-0.4%)的大蒜醇提液剂量依赖性抑制小肠收缩幅度(P<0.05),但对收缩张力无影响(P>0.05);大蒜醇提液抑制小肠收缩幅度的最效浓度为0.2%,小肠收缩幅度仅为用药前26%±7%(P<0.01);0.2%大蒜醇提液与M-受体激动剂氯贝胆碱或KCl联合用药对小肠收缩张力、收缩幅度、峰值和曲线下面积与这两种药物单独用药比差异均不显著(P>0.05)。【结论】大蒜醇提液对小鼠离体小肠平滑肌的运动具有抑制作用,且该抑制作用可能同抑制胆碱能M-受体和阻止细胞内钾离子外流减少无关。

【Objective】To explore the effect of alcohol extract from garlic on gastrointestinal motility and to understand its possible mechanism of action.【Method】In this experiment,the effect of alcohol extract from garlic on the motility of gastrointestinal smooth muscle in mice and its possible mechanism in vitro were monitored by biological signal processing system.【Result】The results showed that garlic alcohol extract with twice concentration(0.025%-0.4%)inhibited the contraction amplitude of small intestine(P<0.05),but the contraction tension had not changed(P>0.05);The maximum effective concentration of garlic was 0.2%which inhibited the contraction of intestine,and the contraction amplitude was 26%±7%of pre-administration(P<0.01);0.2%garlic combined with M-receptor agonist bethanechol chloride or KCl was not significantly different from bethanechol chloride or KCl alone in contraction tension,amplitude,peak and area under curve(P>0.05).【Conclusion】The present results indicate that alcohol extract from garlic inhibits the movement of isolated small intestine in mice,moreover,this inhibition effect may not be related to the inhibition of cholinergic M-receptors and the inhibition of decreased intracellular potassium ion outflow.