电化学阳极氧化处理对3D打印钛合金表面性能的影响

Influence of anodic oxide films on microstructure and properties of 3D printed titanium alloy

目的:研究电化学阳极氧化处理对3D打印钛合金材料表面性能及成骨细胞增殖能力的影响。方法:应用阳极氧化技术对3D打印钛合金(Ti6Al4V)试件及光滑锻造钛合金(Ti6Al4V)试件进行表面处理,构建纳米管形貌,应用场发射扫描电镜、接触角测量仪、表面粗糙度测量仪、X射线光电子能谱对试件表面进行表面形貌、亲水性、粗糙度、结构及晶型测量及分析;在材料表面培养成骨细胞,应用CCK-8法,检测细胞在培养4h及第1,3,5D粘附及增殖情况。结果:应用阳极氧化技术可以在3D打印钛合金表面构建出排列规则有序的纳米管结构,与3D打印材料表面的微米结构相结合,构建复合微纳形貌,该形貌表面为锐钛矿晶型的二氧化钛纳米管,亲水性增加,初步细胞实验结果证实该处理方法促进成骨细胞的粘附增殖。结论:应用阳极氧化技术可以在3D打印钛合金的粗糙制备纳米管结构,构建微纳形貌,该复合形貌提高材料表面亲水性,并促进成骨细胞的粘附及增殖。

Objective: To investigate the effect of electrochemical anodizing on surface properties of 3D printed titanium alloy and the proliferation of osteoblasts. Methods: TiO2 nanotube allays were synthesized on the 3D printed as well as smooth titanium alloy(Ti6Al4V) surfaces through anodic oxidation. The surface topography, roughness, wettability and phage compositions were analyzed by scanning electron microscopy, surface profiler, contact analysis system and X-ray diffraction. Osteoblasts adhesion and proliferation on samples were assessed using CCK-8 assay after culturing for 4 h, 1D,3D, and 5D. Results: The results showed that the anodization process formed regular nanotubular topography on the 3D printed titanium alloy, improved the wettability of the surface without changes of micro-structure. The X-ray diffraction result confirmed the anatase phase of the prepared nanotube layers. The adhesion and proliferation of osteoblasts were improved significantly on the modified surfaces. Conclusion: The unique combination of micro-and nano-scale topography is synthesized on 3D printed titanium alloys through anodic oxidation with increased wettability. The modified surfaces are proposed to improve adhesion and proliferation of osteoblasts.