瑶药五爪金龙中牛蒡苷元薄层色谱及含量测定

Study on TLC and HPLC of Arctigenin in Yao Medicine Ipomoea cairica (L.) Sw.

探索瑶药五爪金龙活性成分牛蒡子苷元的薄层色谱(TLC)定性鉴别和高效液相色谱(HPLC)定量检测方法,寻找瑶药五爪金龙质量控制的依据。本研究采用薄层色谱法对药材中的牛蒡苷元进行定性鉴别,采用高效液相色谱法对药材中的牛蒡苷元进行含量测定。通过实验,确定了TLC定性鉴别和HPLC定量检测的方法。薄层色谱中牛蒡苷元斑点集中、清晰。高效液相色谱法中牛蒡苷元对照品在0.42~5.26μg时,进样量与峰面积呈良好的线性关系。牛蒡苷元平均回收率为100.43%,RSD=2.09%。10批五爪金龙药材中牛蒡苷元含量在0.06%~0.52%。

To explore the TLC qualitative identification and HPLC quantitative detection method of the active component of Yao medicine Ipomoea cairica(L.)Sw.can help to find the basis for the quality control of Yao medicine Ipomoea cairica(L.)Sw.In this research,the arctigenin in herb was qualitatively identified by TLC.The content of arctigenin in herb was determined by HPLC.Through experiments,TLC qualitative identification and HPLC quantitative detection methods were determined.The spots of arctigenin in TLC were concentrated and clear.The injection volume had a good linear relationship with the peak area when the arctigenin reference substance in HPLC is 0.42-5.26μg.The average recovery rate of arctigenin was 100.43%,and RSD was 2.09%.The content of arctigenin in 10 batches of Ipomoea cairica(L.)Sw.was between 0.06%and 0.52%.