铁皮石斛抗疲劳作用的谱效关系研究

Spectrum-effect Relationship between HPLC Fingerprints and Antifatigue Function of Dendrobium officinale

为了探讨铁皮石斛提取物高效液相色谱(HPLC)图谱峰面积与其抗疲劳作用的关联性,为阐明其抗疲劳药效物质基础提供依据。本研究从4个不同产地采集铁皮石斛,分别制取水提取物、醇提取物和制备其HPLC指纹图谱;并开展小鼠负重游泳和游泳疲劳实验,记录力竭游泳时间、检测血清乳酸含量和肝糖原含量,分析抗疲劳作用;最后采用偏最小二乘回归法,研究各提取物指纹图谱共有峰面积与药效的关联性。结果如下:分别制取了各产地铁皮石斛水提取物、醇提取物HPLC指纹图谱,提取出30个特征峰;铁皮石斛水提物、醇提物显著延长小鼠力竭游泳时间、降低游泳疲劳小鼠血清乳酸含量、提高游泳疲劳小鼠肝糖原含量;与延长游泳时间相对应的色谱峰为第4,8,15,23号,与降低血清乳酸含量相对应的色谱峰为第1,3,5,7,9,11,20,24,25,30号,与增加肝糖原含量相对应的色谱峰为第27,28号。实验结果初步证明铁皮石斛抗疲劳作用显著,与延长小鼠力竭游泳时间、降低游泳疲劳小鼠血清乳酸含量或提高游泳疲劳小鼠肝糖原含量相关联的铁皮石斛提取物HPLC色谱峰各不相同。

The correlation between the HPLC fingerprints of Dendrobium offcinale(DO)extracts and its anti-fatigue effects were explored to provide an evidence for clarifying the substances foundation of its anti-fatigue efficacy.In this study,Dendrobium offcinale was collected from four different producing areas,and the water extracts,ethanol extract and HPLC fingerprints chromatography were prepared.The mice were tested for loaded swimming and swimming fatigue,and the exhaustive swimming time was recorded,the serum lactic acid content and liver glycogen content were detected to analyze the anti-fatigue effect.Finally,the partial least squares regression method was used to study the correlation between the common peak area and the efficacy of fingerprints of each extract.The results were as follows:The water extracts of DO from various producing areas and the HPLC fingerprints chromatography of ethanol extract were obtained,and 30 characteristic peaks were extracted.The water extract and ethanol extract of DO significantly prolonged the exhaustive swimming time of mice,reduced the serum lactic acid content in mice with swimming fatigue,and increased the liver glycogen content of mice with swimming fatigue.Peaks of No.4,8,15,23 were related with the effect of DO on prolonging swimming time,peaks of No.1,3,5,7,9,11,20,24,25,30 were related with the effect of DO on decreasing lactic acid,peaks of No.27,28 were related with the effect of DO on increasing the content of liver glycogen.The experimental results preliminarily proved that the anti-fatigue effect of DO was significant,and the HPLC peaks of DO extracts associated with prolonged swimming exhaustion time,decreased serum lactic acid content in swimming fatigue mice,or increased liver glycogen content in swimming fatigue mice were different.