摘要
目的:通过检测KIF2A、LSD1在肾透明细胞癌(RCCC)组织及癌旁非肿瘤组织中的蛋白表达情况探讨其与RCCC发生、发展及生物学行为相关性。方法:运用免疫组织化学方法检测120例肾透明细胞癌组织和40例癌旁非肿瘤组织KIF2A、LSD1蛋白表达情况,分析两者与肾透明细胞癌患者临床病理参数关系。结果:KIF2A蛋白在肾透明细胞癌中的表达(60.83%)显著高于癌旁非肿瘤组织(32.5%),差异有统计学意义(P<0.05),LSD1蛋白在肾透明细胞癌中的表达(55.0%)显著高于癌旁非肿瘤组织(30.0%),差异有统计学意义(P<0.05),KIF2A蛋白在RCCC中的表达与WHO/ISUP病理分级及临床分期相关(P<0.05);LSD1蛋白在RCCC中的表达与WHO/ISUP病理分级相关(P<0.05),与临床分期无关(P>0.05);KIF2A、LSD1蛋白表达与性别、年龄均无关(P>0.05)。相关性检验结果显示:KIF2A、LSD1蛋白表达呈正相关(rs=0.235,P=0.010)。结论:KIF2A和LSD1可能参与了RCCC的发生、发展,并具有协同作用。
Objective: To explore the relationship KIF2 A LDS and the occurrence, development and biological behavior of RCCC by detecting the protein expression of KIF2 A and LSD1 in renal clear cell carcinoma(RCCC) and adjacent non tumor tissues,. Methods: The expression of KIF2 A and LSD1 in 120 cases of renal clear cell carcinoma and 40 cases of adjacent non-tumor tissues to renal clear cell carcinoma were detected by immunohistochemistry. Results: The expression of KIF2 A in renal clear cell carcinoma(60.83%) was significantly higher than that in non-tumor tissues(32.5%). The difference was statistically significant(P<0.05). The expression of LSD1 in renal clear cell carcinoma(55.0%) was significantly higher than that in non-tumor tissues(30.0%). The difference was statistically significant(P<0.05). The expression of KIF2 A in RCCC was significantly higher than that in who/ISUP pathological grade and clinical significance The expression of LSD1 protein in RCCC was correlated with WHO/ISUP pathological grade(P<0.05), but not with clinical stage(P > 0.05). The expression of KIF2 A and LSD1 protein was not correlated with gender and age(P > 0.05). The results of correlation test showed that the expression of KIF2 A and LSD1 was positively correlated(rs = 0.235, P = 0.010). Conclusion: KIF2 A and LSD1 may be involved in the occurrence and development of RCCC and have synergistic effect.
作者
殷凤朝
李芳
刘丽辉
曹赫然
李珅
孙超
YIN Fengchao(Shijiazhuang First Hospital, Hebei Shijiazhuang 050011, China)
出处
《河北医学》
CAS
2019年第12期2049-2053,共5页
Hebei Medicine
基金
河北省医学科学研究重点课题指导计划,(编号:20160798)