关黄柏和川黄柏黄酮含量及抗氧化性的比较分析

Comparative Analysis on Flavonoids Content and Antioxidant Activity of Guan Cortex Phellodendri and Chuan Cortex Phellodendri

采用高效液相色谱法分别测定关黄柏和川黄柏中黄酮含量,采用流动注射化学发光法测定关黄柏和川黄柏的抗氧化性,并对关黄柏和川黄柏中黄酮含量和抗氧化性进行比较分析.高效液相色谱法分别测定关黄柏和川黄柏中黄酮含量的变异系数为0.63%;测得关黄柏的回收率为90.2%~98.4%,川黄柏的回收率为92.4%~106.8%.测得关黄柏中黄酮含量为0.24 mg/g,川黄柏中黄酮含量为0.40 mg/g.流动注射化学发光法测定关黄柏抗氧化性的IC50(抑制率为50%时的溶液的浓度)为0.76mg/mL,川黄柏抗氧化性为0.64 mg/mL.结果可知,川黄柏中黄酮化合物含量大于关黄柏中黄酮化合物含量,川黄柏的抗氧化性亦大于关黄柏的抗氧化性.

High performance liquid chromatography(HPLC)was used to determine the content of flavonoids in Guan Cortex phellodendri and Chuan Cortex phellodendri and flow injection chemiluminescence method was used to determine the antioxidant activity of Guan Cortex phellodendri and Chuan Cortex phellodendri.The coeffi-cient of variation of flavonoids content in Guan Cortex phellodendri and Chuan Cortex phellodendri was 0.63%by HPLC.The recovery rate was 90.2%~98.4%and 92.4%~106.8%respectively.The content of flavonoids in Guan Cortex phellodendri and Chuan Cortex phellodendri was 0.24 mg/g and 0.40 mg/g respectively.The IC50(the concentration of the solution when the inhibition rate was 50%)was 0.76 mg/mL and 0.64 mg/mL re-spectively.The result shows that,The content of flavonoids in Guan Cortex phellodendri was higher than that of Chuan Cortex phellodendri,and the antioxidant activity of Chuan Cortex phellodendri was also higher than that of Guan Cortex phellodendri.