红外热像技术在小鼠阳虚证研究中的运用及其评价体系

Application of infrared thermography in the evaluation of mouse Yangxu Syndrome and its evaluation criteria

目的运用红外热像技术评估糖皮质激素药源性证候小鼠"阳"盛衰程度。方法将120只ICR小鼠分3次实验依次开展,每次实验分成正常对照组以及4个不同剂量糖皮质激素(氢化可的松、泼尼松龙、地塞米松)用药组,在给药第14天拍摄各组小鼠的红外热像图,并尝试分析小鼠头部、侧腹部、尾根部共三个区域的温度情况。结果氢化可的松和地塞米松给药后小鼠头部最高温度、躯干平均温度和尾部最低温度均出现不同程度的下降。泼尼松龙给药后仅仅极低剂量组小鼠头部最高温度出现显著下降。结论红外热像技术可以评估实验小鼠"阳"盛衰程度;氢化可的松和地塞米松可诱发药源性虚证小鼠类似阳虚的外寒征象。

Objective Using infrared thermography to evaluate the rise and fall of Yang in mice with a glucocorticoid drug-induced syndrome. Methods One hundred and twenty SPF male ICR mice were tested in three experiments: each experiment included a normal control group and four groups of different doses of glucocorticoid(hydrocortisone, prednisolone, or dexamethasone), 8 mice in each group. Infrared thermal images of the mice in each group were taken on the fourteenth day of drug administration, and the temperatures at the head, the lateral abdomen and the tail root were analyzed. Results After administration of hydrocortisone and dexamethasone, all the maximum head temperature, the mean trunk temperature and the minimum tail temperature of the mice were decreased to different degrees. After prednisolone administration, the maximum head temperature of mice in the very-low-dose glucocorticoid group showed a significant decrease. Conclusions Infrared thermography can be used to evaluate the degree of "Yang" in experimental mice. Hydrocortizone and dexamethasone can induce cold signs similar to Yang deficiency in mice with drug-induced deficiency syndromes.