天麻芎苓止眩片对自发性与肾性高血压模型大鼠影响的比较研究

Comparison of the effects of Tianma Xiongling Zhixuan Tablet on the blood pressure in spontaneously and renal hypertensive rats

目的探讨天麻芎苓止眩片对自发性高血压大鼠(spontaneously hypertensive rats,SHR)和肾性高血压大鼠(renal hypertensive rats,RHR)大鼠的影响。方法采用RHR和SHR大鼠模型进行实验,连续给药4周,观察比较各周大鼠血压值、左心室指数(left ventricular mass index,LVMI)、生化指标及HE染色观察心脏、肾、脑、主动脉的病理形态变化。结果天麻芎苓止眩片高、中剂量组能显著降低RHR和SHR大鼠的收缩压和舒张压(P<0.01或0.05);低剂量组能够显著降低SHR大鼠的收缩压和舒张压(P<0.01或0.05),但对RHR大鼠无显著的降压效果(P>0.05)。与模型组比,SHR高剂量组左室质量(left ventricular mass,LVM)明显减小,差异具有统计学意义(P<0.05),其他各给药组SHR和RHR大鼠左心室LVM减轻,左心室指数减小,但差异不具有统计学意义(P>0.05)。与RHR模型组比,高剂量组能够显著降低RHR大鼠尿素氮(BUN)、肌酐(CREB)、肌酸激酶(CK)值,中剂量组大鼠能够显著降低乳酸脱氢酶(LDH)、CK、CREA、BUN值,低剂量组能够显著降低大鼠BUN值,差异具有统计学意义(P<0.05或0.01)。与SHR模型组比,对SHR大鼠CK、CREA、CREA、BUN值有一定降低作用,但差异无统计学意义(P>0.05)。结论天麻芎苓止眩片对两种不同高血压大鼠模型均有显著降压效应,具有能够将脉压差保持在合理范围,持续稳定调节血压的作用特点。天麻芎苓止眩片对高血压具有较好的防治药理作用。

Objective To investigate the effects of Tianma Xiongling Zhixuan tablets(TXZT) on the blood pressure of spontaneously hypertensive rats(SHRs) and renal hypertensive rats(RHRs). Methods Both SHRs and RHRs were used in the experiment, and the blood pressures of rats in each group were monitored. The left ventricular index(LVMI) of rats in each group was compared by the weighing method, the serum biochemical indexes were detected by ELISA, and the pathological changes of the heart, kidney, brain and aorta were observed using H&E staining. Results The high and middle doses of TXZT significantly lowered the systolic blood pressure(SBP) and diastolic blood pressure(DBP) of RHRs and SHRs rats(P< 0.01 or 0.05), whereas the low dose TXZT significantly lowered the SBP and DBP of SHRs rats(P< 0.01 or 0.05), but had no significant effect on RHRs rats(P>0.05). Compared with the untreated model group, the LVMI of SHRs rats treated with TXZT was significantly decreased in the high-dose group(P<0.05), but not in other groups(P>0.05). Compared with the RHR model group, the values of creatine kinase(CK), creatinine(CREA) and serum urea nitrogen(BUN) values in the high dose TXZT-treated rats were significantly decreased;Lactate dehydrogenase(LDH), CK, CREA and BUN values were significantly decreased with the middle dose group, and BUN values were significantly decreased with the low dose(P< 0.01 or 0.05). Compared with the SHR model group, the CK, CK, CREA and BUN values were decreased, but the difference was not statistically significant(P>0.05). Conclusions TXZT has good antihypertensive pharmacological effect on the two different rat models of hypertension, can keep the pulse pressure difference in a certain range, and has a sustaining and stable pharmacological action in regulating blood pressure. TXZT has good preventive and therapeutic effects on hypertension.