浙贝黄芩汤调控Wip1基因表达发挥抗小鼠白血病作用

Therapeutic effect of CFSD in a mouse leukemia model through reducing the expression of Wip1

目的明确浙贝黄芩汤体内干预白血病细胞增殖的作用通路,初探浙贝黄芩汤与Wip 1表达水平的相关性。方法尾静脉注射法构建白血病模型小鼠,将其随机为模型组、中药组、化疗组、联合组,另设一组健康小鼠作为空白组。应用浙贝黄芩汤、盐酸多柔比星、及前两者联合对各实验组小鼠进行干预。采用流式细胞技术检测小鼠血液中CD33^+CD117^+细胞比例;定时定量PCR检测Wip 1的表达水平,Western blot检测WIP1蛋白表达含量。结果联合组的白血病细胞CD33^+CD117^+表达最低(P<0.05);模型组小鼠骨髓中Wip 1 mRNA的表达最高,其余各组小鼠骨髓中Wip 1 mRNA表达水平有所降低,(P<0.05)。结论浙贝黄芩汤联合化疗能够提高化疗有效率及白血病疾病缓解率,降低Wip 1 mRNA表达量;浙贝黄芩汤还能在一定程度上保护小鼠肝脾结构的完整性,抑制白血病细胞增殖。

Objective To clarify the role of Compound Fritillaria Scutellaria Decoction(CFSD)as an intervention of leukemia cell proliferation,and to explore the correlation between the administration of CFSD and the expression of Wip 1 in mice.Methods Flow cytometry was used to detect the proportion of CD33 positive cells in the mouse blood.The expression level of Wip 1 was detected by real-time quantitative PCR and the expression of WIP1 protein was detected by Western blot.Results The positive CD33^+CD117^+ leukemia cells of adriamycin plus CFSD(Adr+CFSD)group was the lowest(P<0.01).The expression of Wip 1 mRNA in the bone marrow of model mice was high,and the expression level of Wip 1 mRNA in the bone marrow of the other groups was decreased(P<0.05).Conclusions CFSD combined with chemotherapy improved the efficacy of chemotherapy and the remission rate of leukemia,and reduced the expression of Wip 1 mRNA.CFSD protects the integrity of the liver and spleen structures and partly inhibits the proliferation of leukemia cells.