一些柿属样本遗传多样性的SSAP标记分析

Genetic Diversity Analysis of Diospyros spp. Samples by SSAP Molecular Markers

利用SSAP分子标记对53份柿属样本(26个南方柿、19个北方柿和8个柿属近缘种)开展了遗传多样性分析,并通过扩增产物的条带统计得到了UPGMA聚类图。结果表明:某些地域性柿品种具有相近的亲缘关系,显示出一定的地域特征,但地域之间也存在交错,本研究不能从该聚类图上划分出南方柿与北方柿的分界线;柿品种的起源和进化明显受到了人工选择和商品化交流的影响,栽培品种的遗传多样性趋于狭窄(总体相似系数在0.75以上,多数类群内的相似系数在0.80以上。);探讨柿的起源和进化不能局限在栽培品种上,还应包含宝贵的野生资源和古树资源。

The genetic diversity of 53 Diospyros spp.samples(26 southern persimmons,19 northern persimmons,and 8 related species)was analyzed by SSAP molecular markers,and the UPGMA dendrogram was obtained by statistic analysis of amplified bands.The result showed that some regional varieties had similar genetic relationships and showed certain regional characteristics,but there were also interlaces among regions.The study could not draw a clear line between the northern and the southern samples on the dendrogram.The origin and evolution of persimmon varieties were obviously influenced by artificial selection and commercial exchange.The genetic diversity of cultivated persimmon varieties tends to be narrow(the overall similarity coefficient is above 0.75,and in most groups is above 0.80.).The discussing about the origin and evolution of persimmon should not be confined to cultivated varieties,but should also take the valuable wild resources and ancient tree resources into consideration.