Daxx基因在小鼠睾丸精子发生过程中的表达分析

Expression Analysis of Daxx Gene in Mouse Testis Spermatogenesis

目的:分析Daxx基因在小鼠睾丸精子发生过程中的表达。方法:对不同周龄的野生小鼠的睾丸组织、成年睾丸支持细胞的雄激素受体特异性敲除(SCARKO)以及其雄性激素受体的敲除(ARKO)小鼠睾丸的水平进行检测分析。结果:SCARKO小鼠和野生型的小鼠相比较其睾丸中Daxx基因的表达没有明显不同,然而在生精细胞的细胞核当中呈现极性分布的情况,在ARKO小鼠的睾丸Daxx基因的表达与其他方法相比明显降低。结论:ARKO的小鼠与野生型小鼠相比其Daxx基因的表达明显降低,Daxx基因的定位情况受到睾丸支持细胞中AR基因的特异性敲除的作用。小鼠睾丸精子的发生过程可能有Daxx基因的参与。

Objective: To analyze the expression of Daxx gene in mouse testis spermatogenesis. Methods: The testis tissues of wild mice of different ages, androgen receptor-specific knockout(SCARKO)of adult testicular support cells and the levels of testosterone of their androgen receptor knockout(ARKO) mice were analyzed. Results: There was no significant difference in the expression of Daxx gene between SCARKO mice and wild-type mice. However, the polar distribution of spermatogenic cells was observed in the nucleus of spermatogenic cells, and the expression of Daxx gene in the testis of ARKO mice was different. The method is significantly lower than that. Conclusion: The expression of Daxx gene in ARKO mice is significantly lower than that in wild-type mice. The localization of Daxx gene is specifically knocked out by AR gene in testis-supporting cells.