Box-Behnken响应面法优选蜜炙金银花炮制工艺及其药效学

Optimization of Processing Technology of Honey-stir-baked Lonicerae japonica Flos by Box-Behnken Response Surface Method and Its Pharmacodynamics

目的优化金银花的蜜炙工艺,制定含量测定方法并进行急性咽炎的药效学研究。方法以绿原酸含量为评价指标,选取加蜜量、闷润时间、烘制温度及烘制时间为考察因素,采用Box-Behnken响应面法优化金银花的蜜炙工艺并进行验证实验。采用氨水咽部喷雾的方法建立大鼠急性咽炎动物模型,考察动物血常规指标变化。结果最优蜜炙工艺条件为加蜜量20%,烘制温度154℃,烘制时间13 min,闷润时间1 h。经过优化后的蜜炙金银花可使急性咽炎大鼠咽炎症状减轻,能显著降低小鼠耳部的肿胀,血常规指标值趋于正常。结论优化工艺后的蜜炙金银花质量稳定可控,制备简单可行,为实际生产提供了理论依据。对急性咽炎有较好的治疗作用,扩大了金银花的临床应用范围。

Objective To optimize the honey baking technology of Lonicera japonica,to establish a method for the determination of its content and to study the pharmacodynamics of acute pharyngitis.Methods Taking chlorogenic acid content as the evaluation index,the factors such as honey content,moistening time,baking temperature and baking time were selected as the investigation factors.A rat model of acute pharyngitis was established by means of ammonia spray.And the changes of animal blood routine indexes were investigated.Results The best procedure was as followed:honey content was 20%,moistening time was 1 h,baking temperature was 154℃and baking time was 13 min.After using the optimized honey-stir-baked Lonicera japonica,the symptoms of pharyngitis in acute pharyngitis model were relieved.The results of blood routine assay returned to normal levels,it also significantly reduced the swelling of the ear of mice.Conclusion The quality of honey-stir-baked Lonicera japonica is stable and controllable,and its preparation procedure is simple and feasible,which provides a theoretical basis for practical production.It has good therapeutic effect on acute pharyngitis,which enlarges the clinical application scope of Lonicera japonica.