荔枝DA1同源基因的克隆及生物信息学分析

Cloning and Bioinformatics Analysis of DA1 from Litchi

【目的】克隆荔枝种子大小调控基因DA1,对其进行生物信息学分析。为深入研究荔枝LcDA1基因的功能提供理论参考。【方法】利用生物信息学软件对荔枝DA1同源基因及其编码蛋白进行预测和分析。以荔枝种子cDNA为材料,采用RT-PCR扩增3个LcDA1基因全长。【结果】根据转录组数据获得3个DA1同源基因,分别命名为LcDA1-1、LcDA1-2和LcDA1-3。开放阅读框(ORF)分别为1479,1419和1104 bp,分别编码492,472和367个氨基酸。氨基酸序列分析结果显示3个蛋白均含有典型的UIM和LIM结构域。【结论】荔枝LcDA1蛋白具有典型的DA1保守结构域,可能在荔枝种子发育中具有重要作用。

【Objective】DA1 homologues were identified in litchi and their sequence characteristics were analysed.This paper aimed to provide theoretical reference for studying the function of DA1.【Method】In this study,cDNA from seeds was used to amplify the full sequence of LcDA1.Bioinformatics methods were used to analyze the physical and chemical properties,the structure of LcDA1 proteins.【Result】Based on transcriptomic data,three homologues of DA1 were identified from litchi and named as LcDA1-1,LcDA1-2 and LcDA1-3.Nucleotide sequence of LcDA1-1,LcDA1-2 and LcDA1-3 were predicted to have ORFs of 1479,1419 and 1104 bp,which encode 492,472 and 367 amino acid respectively.amino acid sequence analysis showed that LcDA1-1,LcDA1-2 and LcDA1-3 all contain LIM conserved motifs.【Conclusion】The functionally important domains in LcDA1 proteins are conserved,indicating that LcDA1 proteins play an important role in litchi seed development.