光周期影响罗非鱼脑组织转录组基因表达分析

Photoperiod Effects on Transcriptome Gene Expression in Tilapia Brain Tissue

【目的】发掘尼罗罗非鱼响应光周期变化的重要功能基因,为研究光周期变化对罗非鱼产生影响的分子机制提供参考。【方法】采用新一代高通量测序RNA-seq技术分析24L∶0D、12L∶12D和0L∶24D 3个光周期条件下尼罗罗非鱼脑组织基因表达变化情况。【结果】转录组测序结果显示:3个组别分别产生55524504、61410946和58855762个Clean reads。12L∶12D光周期与0L∶24D光周期文库比较发现279个差异表达基因,12L∶12D光周期与24L∶0D光周期文库比较发现304个差异表达基因,24L∶0D光周期与0L∶24D光周期文库比较发现383个差异表达基因。GO分类分析表明,差异表达基因属于生物学过程、细胞定位和分子功能的42个类别。KEGG通路显著性富集分析差异表达基因共涉及143条代谢途径,包括单纯疱疹感染、ECM-受体相互作用、细胞因子-细胞因子与受体相互作用、肠道IgA生成免疫网络、细胞粘附分子(CAMs)、Wnt信号等通路。【结论】光周期变化影响尼罗罗非鱼脑组织基因表达水平,表达差异基因主要富集在单纯疱疹感染、细胞因子-细胞因子与受体相互作用、Wnt信号通路等信号通路。

【Objective】The study aimed to explore the important functional genes of Nile tilapia in response to photoperiod changes and provide reference for studying the molecular mechanism of photoperiod changes affecting tilapia.【Method】In this study,a new generation of high-throughput sequencing RNA-seq technology was used to analyze the gene expression changes of brain tissue of Nile tilapia under the conditions of 24L∶0D,12L∶12D and 0L∶24D.【Result】Transcriptome analysis showed that 55524504,61410946 and 58855762 clean reads were generated in the threeexperimental groups,respectively.Comparison of 12L∶12D photoperiod with 0L∶24D photoperiod librariesrevealed 279 differentially expressed genes,and comparison of 12L∶12D photoperiod with 24L∶0D photoperiod libraries revealed 304 differentially expressed genes,and comparison of 24L∶0D photoperiod with 0L∶24D photoperiod libraries revealed 383 differentially expressed genes.GO classification analysis revealed that differentially expressed genes existed in 42 categories including biological processes,cellular localization and molecular functions.A total of 143 metabolic pathways were identified in KEGG Pathwaysignificant enrichment analysis,including Herpes simplex infection,ECM-receptor interaction,cytokine-cytokine and receptor interaction,intestinal IgA-generating immune network,cell adhesion molecule(CAMs),Wnt signaling pathway and otherpathways.【Conclusion】Photoperiod changes affect gene expression levels in brain tissue of Nile tilapia.The differentially expressed genes are mainly enriched in signaling pathways such as Herpes simplex infection,Cytokine-cytokine receptor interaction,and Wnt signaling pathway.