摘要
[目的]利用人工胃液和人工肠液对热水提取的桦褐孔菌菌核多糖进行半仿生降解,旨在确定桦褐孔菌菌核多糖在胃液及肠液中的降解方式。[方法]采用苯酚硫酸法、DNS法测定降解过程中桦褐孔菌菌核的糖含量及还原糖含量;应用高效液相色谱法(HPLC)检测降解产物的分子量。[结果]桦褐孔菌菌核多糖在半仿生降解过程中糖含量随降解时间的延长而不断降低,而还原糖含量变化不明显;降解后的桦褐孔菌菌核多糖在HPLC中的保留时间与未降解的桦褐孔菌菌核多糖相比无明显变化,说明降解前后的多糖分子量未发生明显改变。[结论]桦褐孔菌菌核多糖在胃液及肠液中的降解并非从糖链末端、支链的糖苷键逐个断裂,而是通过将多糖的糖链断裂成短链的方式进行降解。
[Objective]To characterize the degradation process of polysaccharides isolated from the sclerotium of Inonotus obliquus in artificial gastric juice and artificial intestinal juice.[Method]The artificial gastric juice and artificial intestinal juice was used to degrade the polysaccharides extracted from the sclerotium of Inonotus obliquus with hot water by using semi-bionic method.The sugar content and the reducing sugar content of the sclerotium polysaccharides during and after the degradation process were determined by the phenol-sulfuric acid method and DNS method,respectively.The molecular weight of the degradation products was assessed by high-performance liquid chromatography(HPLC).[Result]The sugar content of the polysaccharides extracted from the sclerotium of Inonotus obliquus was decreased continuously with the prolonged degradation time in artificial gastric juice and intestinal juice,and the change of reducing sugar content was not significant.The retention time of the degraded sclerotium polysaccharides in HPLC was not significantly different from that of the undegraded sclerotium polysaccharides,which indicated that the molecular weight of polysaccharides had no significant change before and after the degradation.[Conclusion]The polysaccharides isolated from the sclerotium of Inonotus obliquus in artificial gastric juice and artificial intestinal juice degraded neither by breaking the glycosidic bond at the end of the sugar chain nor by breaking that at the branch chain one by one,but by fracturing the sugar chain of the polysaccharides into short chains.
作者
沈子琦
信传鑫
田瑞
苏玲
王琦
SHEN Zi-qi;XIN Chuan-xin;TIAN Rui;SU Ling;WANG Qi(Engineering Research Center of Chinese Ministry of Education for Edible and Medicinal Fungi,Jilin Agricultural University,Changchun 130118,China;College of Plant Protection,Jilin Agricultural University,Changchun 130118,China;Graduate School of Jilin Agricultural University,Changchun 130118,China)
出处
《畜牧与饲料科学》
2019年第12期1-5,共5页
Animal Husbandry and Feed Science
基金
吉林省教育厅“十三五”科学技术项目(JJKH20180648KJ)
中国博士后面上项目(2016M600237)
关键词
桦褐孔菌
菌核多糖
半仿生降解
Inonotus obliquus
polysaccharides from sclerotium
semi-biomimetic degradation