摘要
【目的】从线粒体基因组水平上探讨枣食芽象甲Scythropus yasumatsui与近缘种的系统发育关系。【方法】利用Illumina MiSeq测序平台对枣食芽象甲线粒体基因组进行测序,对基因组序列进行拼装、注释和特征分析;利用贝叶斯法和最大似然法构建基于象甲科13个物种的线粒体基因组13个蛋白质编码基因核苷酸序列的系统发育树。【结果】结果表明,枣食芽象甲线粒体基因组全长为16472 bp(GenBank登录号:MF807224),包含13个蛋白质编码基因、22个tRNA基因、2个rRNA基因和2个非编码控制区,37个基因的排列顺序与祖先昆虫的线粒体基因排列顺序一致。13个蛋白质编码基因的起始密码子为ATN,其中除了cob和nad1基因的完全终止密码子为TAG外,其余11个基因的完全终止密码子为TA(A)。22个tRNA基因中除了trnS1缺少DHU臂,反密码子由GCT变为TCT外,其余均能形成典型的三叶草结构。基于13个蛋白质编码基因序列构建的系统发育树结果显示,象甲科8个亚科系统发育关系为:(((隐喙象亚科(Cryptorhynchinae)+(象虫亚科(Curculioninae)+魔喙象亚科(Molytinae)))+长小蠹亚科(Platypodinae))+(粗喙象亚科(Entiminae)+Cyclominae亚科))+隐颏象亚科(Dryophthorinae)+小蠹亚科(Scolytinae))。【结论】在13种象甲科昆虫物种中,同属于粗喙象亚科的枣食芽象甲与南美果树象甲Naupactus xanthographus在系统发育树中聚为同一分支,表明基于线粒体基因组全序列的分子系统发育结果与传统的形态分类结果是一致的。
【Aim】To explore the phylogenetic relationship between Scythropus yasumatsui and related species at the mitochondrial genome level.【Methods】Using Illumina MiSeq platform,the mitochondrial genome of S.yasumatsui was sequenced,and the genome sequences were assembled,annotated and characterized.Based on the nucleotide sequences of 13 protein-coding genes in the mitochondrial genome,the Bayesian and ML phylogenetic trees of 13 species of the family Curculionidae were constructed.【Results】The results showed that the sequence length of the mitochondrial genome of S.yasumatsui is 16472 bp(GenBank accession no.:MF807224),including 13 protein-coding genes(PCGs),22 tRNA genes,2 rRNA genes and 2 non-coding regions,and the order of genes is consistent with that of the ancestral insect mitochondrial genes.All of the 13 PCGs use ATN as the start codon,and except that cob and nad1 use TAG as the termination codon,other 11 PCGs use TA(A)as the termination codon.All of the 22 tRNA genes were predicted to contain typical cloverleaf secondary structures except the gene trnS1 lacking DHU arm and changing anticodon from GCT to TCT.Phylogenetic tree based on the nucleotide sequences of 13 PCGs showed that the phylogenetic relationship among eight subfamilies of the family Curculionidae was:(((Cryptorhynchinae+(Curculioninae+Molytinae))+Platypodinae)+(Entiminae+Cyclominae))+Dryophthorinae+Scolytinae).【Conclusion】The phylogenetic tree based on the mitochondrial genome showed that among the 13 species of the family Curculionidae,S.yasumatsui and Naupactus xanthographus belonging to the subfamily Entiminae clustered together in the same branch of phylogenetic tree,and this result is consistent with that of traditional morphological classification.
作者
张锋
洪波
王远征
李英梅
陈志杰
ZHANG Feng;HONG Bo;WANG Yuan-Zheng;LI Ying-Mei;CHEN Zhi-Jie(Bio-Agriculture Institute of Shaanxi, Xi′an 710043, China;Enzyme Engineering Research Center of Shaanxi, Xi′an 710043, China)
出处
《昆虫学报》
CAS
CSCD
北大核心
2019年第11期1305-1314,共10页
Acta Entomologica Sinica
基金
陕西省科技统筹创新工程项目(2013KTZB-03-01)
陕西省科学院重大科技项目(2013K-02)