两种提取方法所得秋葵籽油中不饱和脂肪酸成分的对比分析

Comparative Analysis of Unsaturated Fatty Acids in Okra Seed Oil Obtained by Two Extraction Methods

用气相色谱法分别测定用乙醚法和水酶法提取的秋葵籽油,对2种提取方法的黄秋葵籽油分别进行定性和定量分析。研究表明,2种方法提取的不饱和脂肪酸在含量和种类上存在差异。水酶法提取的不饱和脂肪酸有C16:1、C18:1、C18:3(9,12,15)和C20:5共4种,乙醚法提取的不饱和脂肪酸有C18:1、C18:3(9,12,15)、C20:5共3种。水酶法提取的C18:1、C18:3(9,12,15)、C20:5和C22:6的含量分别比乙醚法提取的高11.4 mg·g^-1、2.5 mg·g^-1和2.2 mg·g^-1。

The okra seed oil extracted by ether and enzymatic extraction were determined by gas chromatography.The results showed that the content and types of unsaturated fatty acids extracted by the two methods are different.There were 4 kinds of unsaturated fatty acids extracted by water enzyme method,including C16:1,C18:1,C18:3(9,12,15),C20:5.There were 3 kinds of unsaturated fatty acids extracted by ether method,including C18:1,C18:3(9,12,15),C20:5.The content of C18:1,C18:3(9,12,15),C20:5 extracted by aqueous enzymatic method was 11.4 mg·g^-1,2.5 mg·g^-1 and 2.2 mg·g^-1.