金属硫蛋白1M在非小细胞肺癌中的表达及其与患者预后的关系

Expression of MT1M in non-small cell lung cancer and its relationship with prognosis

目的研究金属硫蛋白1M(metallothionein 1M,MT1M)在非小细胞肺癌(non-small cell lung cancer,NSCLC)组织中的表达及其与肿瘤侵袭、迁移及预后的关系。方法购得144对NSCLC组织和对应癌旁组织的组织芯片,采用免疫组织化学方法检测NSCLC组织中MT1M的表达,分析其与NSCLC患者临床病理参数及预后之间的关系;并在体外细胞Transwell功能实验中分析MT1M对NSCLC细胞侵袭和迁移的作用。结果MT1M在NSCLC组织中的表达明显低于癌旁组织(χ2=143.23,P<0.01),并且NSCLC组织中MT1M的表达与淋巴结转移及TNM分期相关(χ2分别为18.66、12.73,P均<0.01),MT1M抑制NSCLC细胞的侵袭和迁移。结论MT1M在NSCLC组织中异常低表达,其异常低表达可能与NSCLC患者肿瘤转移相关,并且提示NSCLC患者预后不良。

Objective To investigate the expression of metallothionein 1M(MT1M)in nonsmall cell lung cancer(NSCLC)and its relationship with invasion and migration,and further explore the relationship between MT1M and prognosis of NSCLC patients.Methods A total of 144 pairs of NSCLC and corresponding adjacent tissues microarray were purchased.The expression of MT1M detected by immunohistochemistry,and the effects of MT1M on cell invasion and migration were analyzed.Results The expression of MT1M was decreased in cancer tissues relative to corresponding adjacent tissues,the difference was statistically significant(χ2=143.23,P<0.01).MT1M expression in NSCLC was negatively associated with lymph node metastasis(χ2=18.66,P<0.01)and TNM staging(χ2=12.73,P<0.01),MT1M inhibited NSCLC cell invasion and migration.Conclusion MT1M is abnormally low expressed in NSCLC tissues,and its abnormally low expression may be associated with metastasis in NSCLC patients,and suggests poor prognosis in patients with NSCLC.