外源水杨酸（SA）、油菜素内酯（BR）浸种对Ca（NO3）2胁迫下黄瓜幼苗光合特性及叶片解剖结构的影响

Effects of exogenous salicylic acid（SA） and brassinosteroids（BR） soaking on photosynthetic characteristics and leaf anatomical structure of cucumber seedlings under Ca（NO3）2 stress

为了探索外源水杨酸(Salicylic acid,SA)、油菜素内酯(Brassinosteroids,BR)对黄瓜幼苗Ca(NO 3)2胁迫的诱抗作用,以绿岛7号为试材,采用基质培养的方法,研究了外源SA浸种、BR浸种、SA+BR复配浸种对60 mmol/L Ca(NO 3)2胁迫下黄瓜幼苗生长、光合特性和叶片解剖结构的影响。结果表明:外源SA浸种、BR浸种、SA+BR复配浸种均增加了黄瓜幼苗光合色素含量,提高了光合速率,促进了气孔开放,提升了栅栏组织厚度/海绵组织厚度比值。说明SA和BR浸种都能诱导黄瓜幼苗产生耐盐性,缓解Ca(NO 3)2胁迫对黄瓜幼苗生长的抑制作用,其中以SA浸种处理作用效果最明显。

To explore the inhibitory effect of exogenous salicylic acid(SA)and brassinosteroids(BR)on Ca(NO 3)2 stress cucumber seedlings,the effects of exogenous SA soaking,BR soaking and SA+BR soaking on the growth,photosynthetic characteristics and leaf anatomical structure of cucumber seedlings under Ca(NO 3)2(60 mmol/L)stress were studied using Lyudao No.7 as the test material and using the method of matrix culture.The results showed that the treatment of exogenous SA soaking,BR soaking and SA+BR complex soaking increased the photosynthetic pigment content and the photosynthetic rate,promoted stomatal opening,and increased the ratio of palisade tissue thickness to sponge tissue thickness.It indicates that both SA and BR soaking seeds can induce salt-tolerance of cucumber seedlings and alleviate the inhibitory effect of Ca(NO 3)2 stress on the growth of cucumber seedlings.Furthermore,the effect of SA soaking treatment is the most obvious.