摘要
The Na^+/K^+/2Cl^-cotransporter(NKCC)and the cystic fibrosis transmembrane conductance regulator(CFTR)proteins play crucial roles in the transportation of Na^+and Cl^-.In this study,we identified cftr,nkcc1 a,nkcc1 b and nkcc2 in spotted sea bass(Lateolabrax maculatus)genomic and transcriptomic databases.We also characterized these genes via phylogenetic and structural analyses.The results showed that both cftr and nkcc were highly conservative in L.maculatus.Quantitative real-time polymerase chain reaction(qRT-PCR)analysis in ten tissues showed that cftr,nkcc1 a and nkcc2 highly express in osmoregulatory organs such as gill,kidney and intestine.Furthermore,the expressions of cftr and nkcc1 a in gill as well as nkcc2 in intestine were up-regulated by high salinity,indicating that these genes function potentially in osmoregulation.Our findings provided the insights into the cftr and nkcc functions in euryhaline teleost.
The Na^+/K^+/2Cl^- cotransporter(NKCC) and the cystic fibrosis transmembrane conductance regulator(CFTR) proteins play crucial roles in the transportation of Na+ and Cl-. In this study, we identified cftr, nkcc1 a, nkcc1 b and nkcc2 in spotted sea bass(Lateolabrax maculatus) genomic and transcriptomic databases. We also characterized these genes via phylogenetic and structural analyses. The results showed that both cftr and nkcc were highly conservative in L. maculatus. Quantitative real-time polymerase chain reaction(qRT-PCR) analysis in ten tissues showed that cftr, nkcc1 a and nkcc2 highly express in osmoregulatory organs such as gill, kidney and intestine. Furthermore, the expressions of cftr and nkcc1 a in gill as well as nkcc2 in intestine were up-regulated by high salinity, indicating that these genes function potentially in osmoregulation. Our findings provided the insights into the cftr and nkcc functions in euryhaline teleost.
基金
supported by the China Agriculture Research System (No. CARS-47)
the National Natural Science Foundation of China (No. 31602147)
