循环微小RNA-25-3P在骨肉瘤诊断和预后评估中的应用价值

Value of circulating microRNA-25-3P applied to diagnosis and prognostic assessment of osteosarcoma

目的探讨循环微小RNA-25-3P在骨肉瘤诊断和预后评估中的临床价值。方法选择74例骨肉瘤患者作为研究组,57例健康受试者作为对照组。检测两组研究对象的血清微小RNA-25-3P表达水平。采用受试者特征工作曲线评价血清微小RNA-25-3P水平诊断骨肉瘤的效能,采用多因素Cox回归模型分析骨肉瘤患者预后的影响因素。结果研究组血清微小RNA-25-3P相对表达水平为1.29±0.62,低于对照组的4.46±1.37(P<0.05)。血清微小RNA-25-3P诊断骨肉瘤的最佳截断值为2.56,其灵敏度、特异性分别为0.905、0.982,曲线下面积为0.977(P<0.05)。血清微小RNA-25-3P相对表达水平是骨肉瘤患者预后的独立影响因素之一,其≥1.56为骨肉瘤患者术后生存的保护因素(P<0.05)。结论血清微小RNA-25-3P水平对骨肉瘤的诊断和预后评估均有一定临床价值,有望成为诊断骨肉瘤和评估其预后的新标志物。

Objective To investigate the clinical value of circulating microRNA(miR)-25-3P in the diagnosis and prognostic assessment of osteosarcoma.Methods Seventy-four patients with osteosarcoma were selected as study group,and 57 healthy subjects as control group.The serum miR-25-3P expression level was detected in the subjects of both groups.Receiver operating characteristic curve was applied to evaluating the diagnostic efficacy of serum miR-25-3P level for osteosarcoma,and the influencing factors for prognosis in the osteosarcoma patients was analyzed by multivariate Cox regression model.Results The relative expression level of serum miR-25-3P in the study group was lower than that in the control group(1.29±0.62 vs.4.46±1.37,P<0.05).For diagnosis of osteosarcoma,the optimal cut-off value of the serum miR-25-3P was 2.56 with sensitivity of 0.905,specificity of 0.982,and the area under curve of 0.977(P<0.05).The serum miR-25-3P expression level was one of the independent influencing factors for prognosis in the osteosarcoma patients(P<0.05),and the expression level≥1.56 was the protective factor for postoperative survival in the osteosarcoma patients.Conclusion The serum miR-25-3P level obtains a certain clinical value in the diagnosis and prognostic assessment of osteosarcoma,and is expected to be a new marker for diagnosis and prognostic assessment of osteosarcoma.