伤科消肿止痛膏制备工艺以及有效成分含量测定

Preparation technology of the trauma swelling elimination ointment and determination of its active components

目的:优选伤科消肿止痛膏的制备工艺,测定指标成分的含量。方法:设计正交实验优选伤科消肿止痛膏醇提工艺。运用单因素考察法优选制备工艺。采用HPLC色谱法检测大黄素和虎杖苷的含量。结果:优选的醇提工艺为7倍量的70%乙醇提取3次,每次2 h。优选的制备工艺为明胶和阿拉伯胶的比例为1:4,使用3倍量伤科消肿止痛膏提取液,所得膏体黏度适中。测得大黄素的含量为4.14μg·g-1,虎杖苷的含量为12.71μg·g-1。结论:实验优选的工艺稳定可行,可用于伤科消肿止痛膏制备。选取的指标成分大黄素和虎杖苷含量测定结果稳定,可用于质量控制。

Objective:To optimize the preparation technology of trauma swelling elimination and painkiller ointment and to determine the content of its active components.Methods:An orthogonal experiment was designed to optimize the ethanol extraction process.The single factor method was used to optimize the preparation process.The content of emodin and polydatin was determined by HPLC.Results:The best alcohol extraction process was to extract 7 times the amount of 70%ethanol three times,2 hours each.The viscosity of the paste was moderate when the extraction was 3 times the amount and the ratio of gelatin to Arabic gum was 1:4.The content of emodin and polydatin was 4.14μg·g^-1 and 12.71μg·g^-1.Conclusion:The optimized process is stable and feasible,and thus suitable for the preparation of the trauma swelling elimination and painkiller ointment.The index components of emodin and polydatin were stable and feasible,which can be used for quality control.