温阳解郁颗粒对抑郁模型大鼠行为学和血清中单胺类神经递质水平的影响

Effects of Wenyang Jieyu Granules on the Behavior and Serum Level of Monoamine Neurotransmitter in Depression Model Rats

目的:研究温阳解郁颗粒的抗抑郁作用及可能机制。方法:将60只行为、体质量接近的大鼠随机分为空白对照组(蒸馏水)、模型组(蒸馏水)、氟西汀阳性对照组(2.10 mg/kg)和温阳解郁颗粒低、中、高剂量组(1.89、3.78、7.56 g/kg),每组10只;灌胃给药,每天1次,连续28 d。除空白对照组外,其余各组大鼠均在给药同时采用慢性不可预见性温和刺激配合孤养法复制抑郁模型。在给药第0、7、14、21、28天,测定大鼠体质量变化,通过糖水偏好实验和旷场实验测定大鼠的糖水偏好率和4 min内行径总路程、四角路程;末次给药1 h后,通过强迫游泳实验测定大鼠4 min内挣扎状态时间和漂浮状态时间,并采用高效液相色谱法测定大鼠血清中5-羟色胺(5-HT)和多巴胺(DA)含量。结果:与空白对照组比较,模型组大鼠体质量(给药第7、14、21、28天)、糖水偏好率(给药第14、21、28天)显著降低(P<0.05或P<0.01),4 min内行径总路程、四角路程(给药第21、28天)显著减少,4 min内挣扎状态时间显著缩短、漂浮状态时间显著延长(P<0.01),血清中5-HT、DA含量显著降低(P<0.01)。与模型组比较,温阳解郁颗粒各剂量组大鼠体质量(低剂量组在给药第28天,中、高剂量组在给药第21、28天)和糖水偏好率(给药第28天)显著升高(P<0.05或P<0.01),4 min内行径总路程、四角路程(给药第21、28天)显著增加(P<0.01),4 min内挣扎状态的时间显著延长、漂浮状态时间显著缩短(P<0.01),血清中5-HT、DA含量显著升高(P<0.05或P<0.01)。与氟西汀阳性对照组比较,温阳解郁颗粒各剂量组大鼠上述指标差异均无统计学意义(P>0.05)。结论:温阳解郁颗粒对抑郁模型大鼠具有明显的抗抑郁作用,且其作用与氟西汀相当;其机制可能与降低血清中5-HT、DA含量有关。

OBJECTIVE:To study the anti-depressive effects of Wenyang jieyu granules and its possible mechanism.METHODS:A total of 60 rats with similar behavior and body weight were randomly divided into blank control group(distilled water),model group(distilled water),fluoxetine positive control group(2.10 mg/kg)and Wenyang jieyu granules low-dose,medium-dose and high-dose groups(1.89,3.78,7.56 g/kg),with 10 rats in each group.They were given relevant medicine intragastrically,once a day,for consecutive 28 d.Except for blank control group,other groups were given chronic unpredictable mild stress combined with feeding alone to establish depression model.On the 0,7 th,14 th,21 st and 28 th day of administration,the changes of body weight were measured.The sucrose reference test and open-field test were adopted to determined the rate of sucrose reference,total distance and four corner distance within 4 min.1 h after last administration,forced swimming test was used to measure struggling time and floating time within 4 min.The contents of 5-hydroxytryptamine(5-HT)and dopamine(DA)in serum were determined by HPLC.RESULTS:Compared with blank control group,body weight(7 th,14 th,21 st and 28 th day of administration)and the rate of sucrose reference(14 th,21 st and 28 th day of administration)were decreased significantly(P<0.05 or P<0.01);total distance and four corner distance within 4 min(21 st and 28 th day of administration)were decreased significantly;struggling time within 4 min was shortened significantly and floating time within 4 min was prolonged significantly(P<0.01);serum contents of 5-HT and DA were decreased significantly(P<0.01).Compared with model group,body weight(28 th day of administration in low-dose group,21 st and 28 th day of administration in medium-dose group and high-dose group)and the rate of sucrose reference(28 th day of administration)were increased significantly in Wenyang jieyu granules groups(P<0.05 or P<0.01);total distance and four corner distance within 4 min(21 st and 28 th day of administration)were increased significantly(P<0.01);struggling time within 4 min was prolonged significantly and floating time within 4 min was shortened significantly(P<0.01).Serum contents of 5-HT and DA were increased significantly(P<0.05 or P<0.01).Compared with fluoxetine positive control group,there was no statistical significance in above indexes among Wenyang jieyu granules groups(P>0.05).CONCLUSIONS:Wenyang jieyu granule has obvious anti-depression effect on depression model rats,which is similar to fluoxetine,and its mechanism may be related to the down-regulation of serum contents of 5-HT and DA.