基于HPLC指纹图谱及UPLC-Q-TOF-MS法的3种不同来源大黄差异成分研究

Study on Different Components of Rhei Radix Et Rhizoma from 3 Different Origins Based on HPLC Fingerprint and UPLC-Q-TOF-MS

目的:建立不同来源大黄药材的高效液相色谱(HPLC)指纹图谱,并对其中差异成分进行定性鉴别。方法:采用"中药色谱指纹图谱相似度评价系统"(2012版)软件建立30批大黄药材(掌叶大黄20批、唐古特大黄和药用大黄各5批)的HPLC指纹图谱并进行相似度评价,采用偏最小二乘判别法(PLS-DA)结合超高效液相色谱(UPLC)-四极杆-飞行时间串联质谱(Q-TOF-MS)法定性鉴别3种来源大黄的差异性成分。结果:30批大黄药材样品指纹图谱的相似度在0.609~0.960之间。经PLS-DA分析,大黄药材样品按来源明显聚集为3组,3组样品间差异性成分共有18个,经UPLC-Q-TOF-MS鉴别为白藜芦醇-4′-O-β-D-(6″-O-没食子酰)-葡萄糖苷、莲花掌苷、大黄酸-8-O-葡萄糖苷、表儿茶素没食子酸酯、4-(4′-羟苯基)-2-丁酮-4′-O-β-D-(2″-O-肉桂酰基-6″-O-没食子酰基)-葡萄糖苷等。结论:建立的方法可有效区分3种不同来源大黄药材,并对其差异成分进行鉴别,为多来源药材的鉴别和质量评价提供了参考。

OBJECTIVE:To establish HPLC fingerprints of Rhei Radix Et Rhizoma from different origins,and identify the differential components qualitatively.METHODS:HPLC fingerprints of 30 batches of Rhei Radix Et Rhizoma(20 batches of Rheum palmatum,5 batches of Rheum tanguticum and 5 batches of Rheum officinale)were established and similarity evaluation was performed by using Similarity Evaluation System for Chromatographic Fingerprint of TCM(2012 edition).Qualitative identification of differential components of Rhei Radix Et Rhizoma from 3 different origins were performed by using PLS-DA combined with UPLC-Q-TOF-MS.RESULTS:The fingerprint similarities of 30 batches of samples were between 0.609 and 0.960.According to PLS-DA analysis,Rhei Radix Et Rhizoma were significantly aggregated into 3 groups according to the origin.There were 18 different components among 3 groups,which were identified by UPLC-Q-TOF-MS as resveratrol-4′-O-β-D-(6″-O-gallacyl)-glucoside,lindleyin,rhein-8-O-glucoside,epicatechin gallate,4-(4′-hydroxyphenyl)-2-butanone-4′-O-β-D-(2″-O-cinnamyl-6″-O-gallacyl)-glucoside.CONCLUSIONS:Established method can effectively identify Rhei Radix Et Rhizoma from 3 different origins,and the differential components can be distinguished,which provides a reference for the identification and quality evaluation of multi-source medicinal materials.