消癌平对肝癌细胞HepG2差异表达基因及可变剪接的影响

Effects of Xiaoaiping on the differentially expressed genes and differentially splicing in HepG2 cell line

目的通过RNA-seq分析消癌平作用前后肝癌细胞HepG2的差异表达基因(differentially expressed genes,DEG)及可变剪接(alternative splicing,AS)。方法本研究以HepG2细胞作为对照组,以质量浓度为40 mg/L消癌平作用24 h后的肝癌细胞为实验组,利用Illumina HiSeq平台对实验组和对照组进行分析,使用PossionDis方法进行DEG检测,使用rMATS软件检测不同样品间的AS和差异剪接基因(differentially spliced genes,DSG),并利用基因本体(gene ontology,GO)对其功能进行分类及富集分析。结果与对照组比较,消癌平作用后共得到DEG 608个,其中上调基因147个,下调基因461个。对照组发生AS 21387个,消癌平使HepG2 AS减少到19265个,且两组均以外显子(SE)跳跃最多而内含子(RI)保留最少为主。对差异表达的AS行GO富集分析,提示其中生物过程相关的22个,细胞成分相关13个,分子功能相关的有8个。结论消癌平诱导HepG2产生的大量DEG及AS在抑制肝癌生长增殖过程中发挥作用。

Objective To investigate the effect of Xiaoaiping on the differentially expressed gene(DEG)and the alternative splicing(AS)in HepG2.Methods HepG2 cell lines were resurrected and amplified in vitro.The differentially DEG in HepG2 with or without the treatment of Xiaoaiping were determined on Illumina HiSeq Platform.rMATS was used to detect the AS and differentially spliced genes(DSG)among samples.Gene ontology(GO)classification and functional enrichment were performed.Results Compared with control group,after the action of Xiaoaiping,there were 608 DEG.There were upregulated 147 genes and 461 downregulated genes.19265 and 21387 AS were recognized in experimental group and control group.GO classification and functional enrichment showed that 22 AS events involved in biological process,13 AS events involved in cellular component and 8 AS events involved in molecular function.Conclusion Xiaoaiping resulted a large number of DEGs and AS in HepG2.