miR-1269在胃癌及胃黏膜癌前病变组织中表达及其临床意义

Expression of miR-1269 in gastric cancer and gastric precancerous lesions and its clinical significance

目的探讨胃癌及胃黏膜癌前病变组织中miR-1269的表达及其临床意义。方法选取2016年1月至2018年2月郑州大学第五附属医院收治的50例胃癌患者、50例胃黏膜癌前病变患者及50例浅表性胃炎患者作为研究对象,采用RT-PCR法分别检测胃癌组织、胃黏膜癌前病变组织及浅表性胃炎组织标本中miR-1269的表达,并分析其表达与胃癌临床病理特征的相关性。结果miR-1269在胃癌组织中的相对表达量明显高于胃黏膜癌前病变组织及浅表性胃炎组织,差异有统计学意义(P<0.05);miR-1269在不同分化程度、TNM分期、浸润程度、是否发生淋巴结转移的胃癌组织中的表达量比较,差异有统计学意义(P<0.05),即分化程度越低、TNM分期越高、浸润深度越深、发生淋巴结转移,则胃癌患者癌组织中miR-1269的相对表达量越高;ROC曲线分析结果显示,miR-1269诊断胃癌的AUC为0.807(95%CI:0.614~1.829),诊断灵敏性为88.21%,特异性为79.46%。在完成随访的48例患者中,miR-1269高表达19例,其中死亡8例(42.11%),miR-1269低表达29例,死亡4例(13.79%),两组死亡率比较差异有统计学意义(χ^2=4.907,P=0.027)。由生存曲线可知,高表达组胃癌患者的平均生存时间明显短于低表达组,平均生存时间分别为16.5个月和23.7个月。结论miR-1269在胃癌组织中表达上调,其表达水平与胃癌的发生、发展及预后有关,其可能成为胃癌临床诊疗及预后评估的生物学标志物。

Objective To investigate the expression and clinical significance of miR-1269 in gastric cancer and gastric precancerous lesions.Methods From Jan.2016 to Feb.2018,50 patients with gastric cancer admitted to the Fifth Affiliated Hospital of Zhengzhou University,50 patients with gastric mucosal precancerous lesions and 50 patients with superficial gastritis were selected as subjects.RT-PCR was used to detect the expression of miR-1269 in gastric cancer tissues,gastric precancerous lesions and superficial gastritis tissue samples,and the correlation between the expression of miR-1269 and clinicopathological features of gastric cancer was analyzed.Results The relative expression of miR-1269 in gastric cancer tissues was significantly higher than that in gastric precancerous lesions and superficial gastritis(P<0.05).The expressions of miR-1269 in different degrees of differentiation,TNM stage,degree of infiltration,and lymph node metastasis in gastric cancer were significantly different(P<0.05),the lower degree of differentiation,the higher TNM stage,the deeper infiltration depth,lymph node metastasis,the higher relative expression of miR-1269 in gastric cancer.The ROC curve analysis showed that the AUC of miR-1269 in the diagnosis of gastric cancer was 0.807(95%CI:0.614-1.829),the diagnostic sensitivity was 88.21%,and the specificity was 79.46%.Among the 48 patients who completed the follow-up,19 patients had higher expression of miR-1269,and 8 patients(42.11%)died,29 patients had lower expression of miR-1269,and 4 patients(13.79%)died.There was statistically significant difference in mortality between two groups(χ^2=4.907,P=0.027).From the survival curve,the average survival time of patients in higher expression of gastric cancer was significantly shorter than that in lower expression group,and the average survival times were 16.5 months and 23.7 months,respectively.Conclusion miR-1269 is up-regulated in gastric cancer,and the expression of miR-1269 is related to the occurrence and development and prognosis of gastric cancer,which may become a biomarker for clinical diagnosis and prognostic evaluation of gastric cancer.